改良UW灌注液治疗新生猪急性肾小管坏死的实验研究

目的 通过改良UW(University of Wisconsin solution)肾保存液在体肾动脉灌注,探讨改良UW肾保存液对新生猪急性肾小管坏死的治疗作用.方法 出生1周无特殊病原体幼猪10只,通过夹闭肾动脉制作急性肾小管坏死动物模型,将其分为模型组和改良UW灌注治疗组,每组5只,另设5只为假手术组.分别记录术后各组幼猪12 h、1 d、2 d、3 d、7 d的尿量并进行尿蛋白定量及静脉血中尿素氮(BUN)和肌酐(Cr)含量的检测,同时于术后12 h、24 h及7 d行肾脏病理学检查.结果 10只幼猪经肾脏病理和血生化检查证实建模成功,术后12 h模型组和灌注治疗组BUN和Cr含量明显高于假手术组,差异有统计学意义(P＜0.05),其含量在第2天达到峰值,第7天均显著降低,以灌注治疗组最为显著,并恢复至假手术组水平.尿蛋白定量模型组和灌注治疗组与假手术组比较各时间段均明显升高,差异有统计学意义(P＜0.05或P＜0.01),1 d后达到峰值,后缓慢下降,灌注治疗组下降尤为明显.尿量在模型组各时间段均较假手术组显著减少(P＜0.05或P＜0.01);而灌注治疗组只在12 h、1 d、2 d减少,差异有统计学意义(P＜0.05),在3 d、7d则无统计学意义.肾脏病理学检查提示:与模型组相比灌注治疗组的病理学改变较模型组明显减轻.结论 肾动脉灌注改良UW肾保存液可能具有缓解新生猪急性肾小管坏死程度的作用.

Abstract：

Objective To determine the effect of the modified UW (University of Wisconsin)solution in the treatment of acute renal tubular necrosis in newborn swine. Methods Ten one-week-old newborn swine were used to establish the animal model of acute renal tubular necrosis by clamping their renal arteries,and were divided into two groups: the model group( n = 5 ) and the treatment group ( n = 5 ) in which fructose diphosphate sodium UW solution was used. Sham surgery was performed on other five swine, which were used as the sham group. At 12 h,l d,2 d,3 d and 7 d after the operation,the urine volume,urine protein,blood urea nitrogen(BUN) and creatinine(Cr) were determined. At 12 h ,24 h and 7 d after the operation ,renal pathological examination was conducted. Results The renal pathological examination and the blood biochemistry tests showed that the animal model was successful. BUN and Cr in the model group and the treatment group were significantly higher than those in the sham group at 12 h after operation(P ＜0. 05) ,and they arrived at their peak values at 2 d after operation,showed remarkable decline at 7 d,especially in treatment group,and returned to the level of the sham group. The urine protein in the model group and treatment group were higher than those in the sham group at various times(P ＜0.05 or P ＜0.01) and it peaked at 1 d after operation,then declining gradually,especially in the treatment group. Compared with the sham group,there were a significant decrease in the urine volume at various times in the model group(P ＜0. 05 or P ＜0. 01 ) ,while in the treatment group,the decrease in the urine volume were significant only at 12 h, 1 d and 2 d( P ＜ 0. 05 ) ,and turned insignificant at 3 d and 7 d. The pathological examination showed that the pathological changes in the treatment group were significantly milder than those in the model group. Conclusion The modified UW solution is effective in reducing the acute renal tubular necrosis in newborn swine.

Experimental study on the effect of modified UW solution in the treatment of acute renal tubular necrosis in newborn swine

Objective To determine the effect of the modified UW (University of Wisconsin)solution in the treatment of acute renal tubular necrosis in newborn swine. Methods Ten one-week-old newborn swine were used to establish the animal model of acute renal tubular necrosis by clamping their renal arteries,and were divided into two groups: the model group( n = 5 ) and the treatment group ( n = 5 ) in which fructose diphosphate sodium UW solution was used. Sham surgery was performed on other five swine, which were used as the sham group. At 12 h,l d,2 d,3 d and 7 d after the operation,the urine volume,urine protein,blood urea nitrogen(BUN) and creatinine(Cr) were determined. At 12 h ,24 h and 7 d after the operation ,renal pathological examination was conducted. Results The renal pathological examination and the blood biochemistry tests showed that the animal model was successful. BUN and Cr in the model group and the treatment group were significantly higher than those in the sham group at 12 h after operation(P ＜0. 05) ,and they arrived at their peak values at 2 d after operation,showed remarkable decline at 7 d,especially in treatment group,and returned to the level of the sham group. The urine protein in the model group and treatment group were higher than those in the sham group at various times(P ＜0.05 or P ＜0.01) and it peaked at 1 d after operation,then declining gradually,especially in the treatment group. Compared with the sham group,there were a significant decrease in the urine volume at various times in the model group(P ＜0. 05 or P ＜0. 01 ) ,while in the treatment group,the decrease in the urine volume were significant only at 12 h, 1 d and 2 d( P ＜ 0. 05 ) ,and turned insignificant at 3 d and 7 d. The pathological examination showed that the pathological changes in the treatment group were significantly milder than those in the model group. Conclusion The modified UW solution is effective in reducing the acute renal tubular necrosis in newborn swine.