| 自身免疫性心肌炎心肌基质金属蛋白酶的其抑制因子表达改变的意义 |
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| 引用本文： | 熊然,唐其柱,魏小红,沈涤非. 自身免疫性心肌炎心肌基质金属蛋白酶的其抑制因子表达改变的意义[J]. 心肺血管病杂志, 2011, 30(1): 53-57. DOI: 10.3969/j.issn.1007-5062.2011.01.016 |
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| 作者姓名： | 熊然 唐其柱 魏小红 沈涤非 |
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| 作者单位： | 1. 首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所,心内科CCU,北京,100029
2. 武汉大学人民医院,心内科 |
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| 摘 要： | 目的:以人工合成多肽诱导的实验性自身免疫性心肌炎(experimental autoimmune myocar-ditis,EAM)小鼠为实验模型,分析心肌内基质金属蛋白酶(matrix metalloproteinases,MMPs)及组织基质金属蛋白酶抑制因子(tissue inhibitors of matrix mentalloproteinases,TIMPs)表达变化,探讨病理性自身免疫可能的致病机制。方法:人工合成多肽诱导易感品系(BALB/C)小鼠构建EAM模型。超声评价2组小鼠血流动力学变化情况。分别以免疫组化法、逆转录多聚酶链反应(RT-PCR)法,比较2组小鼠心肌组织中MMP-3、MMP-9及TIMP-1在蛋白质水平与基因水平的表达变化。Masson三色染色法分析2组心肌胶原含量情况。结果:病理切片观察模型组小鼠心肌炎症反应明显。超声检查结果显示,模型组较对照组心功能明显恶化。免疫组化与RT-PCR结果分析显示,模型组MMP-3、MMP-9蛋白质及基因表达水平明显高于对照组,而TIMP-1的蛋白质及基因表达水平均相对下降。Masson染色切片分析显示,2组心肌胶原含量差异无统计学意义。结论:EAM模型小鼠心肌组织MMPs表达水平增高,而TIMPs表达水平下降,2者表达比例失衡。该失衡状态可能影响心肌胞外基质的正常功能,并进一步影响心功能。
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| 关 键 词： | 心肌炎 自身免疫 组织基质金属蛋白酶抑制因子 动物试验,小鼠 |
Effects and significances of the balance between MMPs and TIMPs in experimental autoimmune myocarditis mice model |
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| XIONG Ran,TANG Qizhu,WEI Xiaohong,SHEN Difei. Effects and significances of the balance between MMPs and TIMPs in experimental autoimmune myocarditis mice model[J]. Journal of Cardiovascular and Pulmonary Diseases, 2011, 30(1): 53-57. DOI: 10.3969/j.issn.1007-5062.2011.01.016 |
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| Authors: | XIONG Ran TANG Qizhu WEI Xiaohong SHEN Difei |
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| Affiliation: | XIONG Ran,TANG Qizhu,WEI Xiaohong,SHEN Difei Department of CCU,Capital Medical University affiliated Beijing Anzhen Hospital,Beijing Institute of Heart,Lung and Blood Vessel Diseases,Beijing 100029,China |
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| Abstract: | Objective:Using the experimental autoimmune myocarditis BALB/C mouse model(EAM),which was induced with peptide antigen derivatized from cadiac myosin,as the tool for investigating the change of cardiac extracellular matrix in experimental autoimmue myocarditis.To analysis the alteration of MMPs and TIMPs expression in cardiac extracellular matrix of the EAM model and the impact of this phenomenon.Meth-ods:Thirty-two eight-week-old BALB/C mice were divided into two groups.Peptide antigen was synthesized by m... |
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| Keywords: | Myocarditis Autoimmune Matrix metalloproteinases Tissue inhibitors of matrix mental-loproteinases Animals labroratory mice |
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