鼠脑损伤早期脑皮质微血管D2受体活性变化的实验研究

 目的 通过大鼠的脑损伤模型,检测颅脑损伤后30 min～48 h脑皮质微血管中D<,2>受体活性的变化及其与脑水肿的关系.方法 用改良的Feeney脑落体撞击伤方法制作脑损伤模型;用干-湿法测脑组织水含量;用同位素标记法测定受体变化;用D<,2>受体阻滞剂Sulpiride进行实验治疗.结果 脑损伤30 min后D<,2>受体显著增加,至24 h降至最低,48 h仍未恢复正常.结论 D<,2>受体活性变化在外伤性脑水肿的发生、发展中起重要作用.

Experimental study on the change of D2 receptor activities in cerebral cortical microvessels following early brain injury

Objective To invetigate the change of D 2 receptor activities in cerebral cortical microvessels and its relationship with secondary brain edema following early stage of brain injury.Methods Brain injury model was induced by modified Feeney's method.The measurement of dopaminergic receptors was made by using radio-labelling assay.The water content of brain tissue was measured by dry-wet method.The dopaminergic receptor blocking agent,Sulpiride,was selected to effect the changes of dopaminergic receptor and traumatic brain edema.Results The dopaminergic receptor increased remarkably at 0.5 h after the injury,then fell to the nadir at 24 h , and was still at low level at 48 h as compared with the control group. Conclusions The change of D 2 receptor actioity in cerebral cortical miciovessels might play an important role in the secondary edema of injured brain.