中药复方合剂减轻大鼠心肌梗死后心肌纤维化

目的探讨中药复方合剂能否减轻心肌梗死后心肌纤维化。方法通过结扎冠状动脉前降支制造大鼠心肌梗死模型。将60只SD大鼠分为六组:14天和56天非治疗组,14天和56天治疗组,14天和56天假手术组。模型制作24h后,14天和56天治疗组开始灌胃给药。结果假手术组、非治疗组、治疗组在第14天及第56天时,体重差异无显著性(P>0.05)。14天非治疗组全心重、左心室重量、全心重/体重、左心室重量/体重与14天假手术组差异均无显著性(P>0.05),而56天非治疗组上述各项指标(分别为1054±22mg,850±21mg,2.77±0.08,2.23±0.04)均显著高于56天假手术组(分别为990±14mg,786±14mg,2.58±0.04,2.05±0.03)(P<0.01)。14天治疗组上述各项指标(分别为778±13mg,607±11mg,2.54±0.04,1.98±0.03)显著低于14天非治疗组(分别为802±14mg,630±16mg,2.69±0.07,2.10±0.06)(P<0.01),56天治疗组上述各项指标(分别为1031±14mg,822±14mg,2.65±0.04,2.11±0.02)显著低于56天非治疗组(P<0.01),但高于56天假手术组(P<0.01)。治疗组第14天及第56天心肌胶原含量(分别为4.37±0.57mg/g,6.04±0.69mg/g)均显著低于非治疗组(分别为5.22±0.52mg/g,4.37±0.57mg/g)(P<0.01),但仍高于假手术组(分别为3.65±0.41mg/g,4.33±0.51mg/g)(P<0.01)。心肌组织病理学检查发现治疗组心肌间质纤维化明显减少。结论中药复方合剂有明显的抑制心肌胶原增生的作用,可减轻心肌梗死后心肌纤维化程度。

The Experiment Study of the Traditional Chinese Herbal Compound Reverse Myocardial Fibrosis after Acute Myocardial Infarction

Aim To study traditional Chinese herbal compound reverse myocardial fibrosis after acute myocardial infarction (AMI) in rats. Methods AMI model was established by ligature of left anterior descending coronary(LAD). All of the models were divided into 6 groups at random with ten in every group: group M_ 14 (without any treatments, killed at the 14 th day after infarction), group M_ 56 (without any treatments, killed at the 56th day after infarction), group T_ 14 (treated with herbal compound for 14 days before kill), group T_ 56 (treated with herbal compound for 56 days before kill), S_ 14 (sham operation, killed at the 14th day after surgery), and group S_ 56 (sham operation, killed at the 56th day after surgery) in 60 male SD rats. 24 hours after operation, the herbal compound (10 mL/kg·day) was given for 14 days or 56 days. Results Body weight (BW) of all the rats had non-significant difference (p<0.05). There were not significant differences in heart weight (HW), left ventricle weight (LVW), HW/BW, LVW/BW between group M_ 14 and S_ 14 (p<0.05), but these indexes in group M_ 56 (separate 1 054±22 mg, 850±21 mg, 2.77±0.08, 2.23±0.04) were higher than those in group S_ 56 (990±14 mg, 786±14 mg, 2.58±0.04, 2.05±0.03)(p<0.01). HW, LVW, HW/BW, LVW/BW in T groups were lower at the 14 th and the 56th day (separate T_ 14 778±13 mg,607±11 mg, 2.54±0.04, 1.98±0.03; T_ 56 1 031±14 mg, 822±14 mg, 2.65±0.04, 2.11±0.02) than those in M groups at corresponding time (p<0.01). Collagen contents of T groups (separate T_ 14 4.37±0.57 mg/g, T_ 56 6.04±0.69 mg/g) were lower at the 14th and 56th day than those of M groups (separate M_ 14 5.22±0.52 mg/g, M_ 56 4.37±0.57 mg/g) (p<0.01), but were still higher than those of S group (separate S_ 14 3.65±0.41 mg/g, S_ 56 4.33±0.51 mg/g)(p< 0.01 ). A great deal of collagen fibers deposit in infarct zone and non-infarct zone and collagen deposition declined obviously after treatment of herbal compound under histopathologic observation. Conclusion Herbal compound can reverse myocardial fibrosis to some extent.