摩罗丹（浓缩丸）对胃黏膜损伤保护及镇痛抗炎止血作用研究

目的：评价摩罗丹（浓缩丸）镇痛、抗炎、止血以及对大鼠胃黏膜损伤的保护作用，进一步补充摩罗丹（浓缩丸）在药效学方面的实验研究。方法：通过测定吲哚美辛、盐酸-乙醇致大鼠胃黏膜损伤的溃疡指数，评价摩罗丹（浓缩丸）对胃黏膜损伤的保护作用；通过小鼠腹腔毛细血管通透性实验，评价摩罗丹（浓缩丸）的抗炎作用；通过醋酸致小鼠扭体实验，评价摩罗丹（浓缩丸）的镇痛作用；通过测定小鼠出血、凝血时间，评价摩罗丹（浓缩丸）的止血作用。结果：吲哚美辛致大鼠胃黏膜损伤模型中，摩罗丹（浓缩丸）高剂量、中剂量、低剂量组和阳性药枸橼酸铋钾颗粒组的溃疡指数与模型对照组比较明显降低（P<0.01）；盐酸-乙醇致大鼠胃黏膜损伤模型中，摩罗丹（浓缩丸）高剂量、中剂量组和阳性药枸橼酸铋钾颗粒组的溃疡指数与模型对照组比较明显降低（P<0.05）；摩罗丹（浓缩丸）高剂量、中剂量组和阳性药云南白药胶囊组出血时间明显低于空白对照组（P<0.05）；摩罗丹（浓缩丸）高剂量组和阳性药云南白药胶囊组凝血时间与空白对照组比较明显降低（P<0.05）；醋酸致小鼠扭体反应实验中，摩罗丹（浓缩丸）高剂量组和阳性药布洛芬颗粒组的扭体次数明显低于模型对照组（P<0.05）；小鼠腹腔毛细血管通透性实验中，摩罗丹（浓缩丸）高剂量、中剂量组和阳性药布洛芬颗粒组的腹腔液吸光度明显低于模型对照组（P<0.05）。结论：摩罗丹（浓缩丸）对吲哚美辛等非甾体抗炎药、乙醇刺激引起的胃黏膜破损、溃疡具有治疗作用，对慢性非萎缩性胃炎中常见的糜烂出血、疼痛、炎症也同样具有治疗作用，并能有效保护胃黏膜。

Anti-inflammatory,Analgesic,and Hemostatic Effects of Moluodan(Condensed Pills) and Its Inhibitory Activity on Gastric Mucosal Injury

To evaluate the anti-inflammatory,analgesic,and hemostatic effects of Moluodan(Condensed Pills) and its inhibitory activity on gastric mucosal injury and further launch the experimental research on the pharmacodynamics of Moluodan(Condensed Pills).Methods:The protective effect of Moluodan(Condensed Pills) against gastric mucosal injury was evaluated by measuring the ulcer index of gastric mucosal injury in rats induced by indomethacin and hydrochloric acid-ethanol.The anti-inflammatory effect of Moluodan(Condensed Pills) was evaluated through the peritoneal capillary permeability test in mice.The analgesic effect of Moluodan(Condensed Pills) was evaluated by the acetic acid-induced writhing test in mice.The hemostatic effect of Moluodan(Condensed Pills) was evaluated by measuring the bleeding time and coagulation time of mice.Results:In the gastric mucosal injury model in rats induced by indomethacin,the ulcer indexes of high-,medium-,and low-dose Moluodan(Condensed Pills) groups and the positive drug group colloidal bismuth subcitrate(CBS) granules] were significantly lower than that of the model control group(P<0.01).In the gastric mucosal injury model in rats induced by hydrochloric acid-ethanol,the ulcer indexes of high-and medium-dose Moluodan(Condensed Pills) groups and the positive drug group were significantly lower than that of the model control group(P<0.05).The bleeding time of high-and medium-dose Moluodan(Condensed Pills) groups and the positive drug group(Yunnan Baiyao Capsules) was significantly shorter than that of the blank control group(P<0.05).The coagulation time of the high-dose Moluodan(Condensed Pills) group and the positive drug group was significantly shorter than that of the blank control group(P<0.05).In the writhing test in mice induced by acetic acid,the writhing times in the high-dose Moluodan(Condensed Pills) group and the positive drug group(ibuprofen) was significantly less than that in the model control group(P<0.05).In the peritoneal capillary permeability test in mice,the absorbance of peritoneal fluid in the high-and medium-dose Moluodan(Condensed Pills) groups and the positive drug group(ibuprofen granules) was significantly lower than that in the model control group(P<0.05).Conclusion:Moluodan(Condensed Pills) has a therapeutic effect on gastric mucosal injury and ulcers caused by alcohol stimulation,indomethacin,and other non-steroidal anti-inflammatory drugs.It also has a therapeutic effect on erosion,bleeding,pain,and inflammation common in chronic non-atrophic gastritis,and can effectively protect the gastric mucosa.