Affiliation: | 1. Centre de Recherche en Cancérologie de Lyon, CNRS UMR5286, Inserm U1052, Université Lyon 1, Université de Lyon, Lyon, France;2. Inserm 1035, University of Bordeaux, Bordeaux, France;3. ENS de Lyon, CNRS, Université Lyon 1, Institut de Génomique Fonctionnelle de Lyon, UMR 5242, Université Lyon, Lyon Cedex 07, France;4. ENS de Lyon, CNRS, Université Lyon 1, Institut de Génomique Fonctionnelle de Lyon, UMR 5242, Université Lyon, Lyon Cedex 07, France SFR Biosciences, ENS de Lyon, Inserm US8, CNRS UMS3444, Univ Lyon, Lyon, France;5. BioMeca, Lyon, France |
Abstract: | Human skin is particularly vulnerable to age-related deterioration and undergoes profound structural and functional changes, reflected in the external skin appearance. Skin ageing is characterized by features such as wrinkling or loss of elasticity. Even if research advances have been done concerning the molecular mechanisms that underlie these changes, very few studies have been conducted concerning the structure stiffness of the skin organ as a whole. In this study, we showed, thanks to human skin reconstructs and the Japanese Medaka fish model, that biomechanics is a new biomarker of skin ageing. We revealed that global stiffness measurement by Atomic Force Microscopy, since modulated through ageing in these models, can be a new biomarker of skin ageing, and reflects the profound reorganization of the dermis extracellular matrix, as shown by Transmission Electron Microscopy. Moreover, our data unveiled that the Japanese Medaka fish could represent a highly relevant integrated model to study skin ageing in vivo. |