EGF对TPN大鼠肠粘膜细胞EGFR表达的影响

利用大鼠全肠外营养(TPN)模型,探讨表皮生长因子(EGF)对TPN支持后肠粘膜细胞表皮生长因子受体(EGFR)表达的影响。方法:48只SD大鼠随机分为对照组、常规TPN组及TPN-EGF组。实验持续14天,采用免疫组化结合计算机图象分析的方法,分别测定颌下腺内EGF含量以及小肠粘膜细胞中EGF和EGPR含量。结果:TPN及TPN-EGF组大鼠颌下腺内EGF含量均明显下降(P<0.01);TPN组小肠粘膜细胞中EGF、和EGFR含量显著下降(P<0.01),而TPN-EGF组则未减少(P<0.05)。结论:内源性EGF分泌减少是TPN肠粘膜屏障损伤的重要原因之一;EGF具有促进肠粘膜细胞EGFR表达的作用。

The Influence of EGF on the Expression of EGFR in the Intestinal Cells of Rats Receiving TPN.

To study the influence of EGF on the expression of EGFR in the intestinal cells of rats re-ceiving TPN. Methods: Forth-eight Sprague Dowley rats were randomly divided into control, TPN and TPN-EGF groups. The experimentation lasted for 14 days. EGF and EGFR were studied im-munohistochemically and quantitated by using the image processing and analysis system. Results: EGF content in the submaxillary gland was decreased(P<0.01) in both the TPN and the TPN-EGF groups. TPN induced a marked reduction in the EGF content of intestinal cells(P<0.01), but the EGF content of the intestinal cells remained unchanged(P<0.05) when EGF was administered along with TPN. The EGFR expression(P<0.01) in the intestinal mucosa, bore no significant difference between the TPN-EGF group and the control group. Conclusions: Reduction in endogenous EGF secretion is one of the most important causes leading to intestinal atrophy in TPN rats. EGF might enhance the expression of EGFR in the intes-tinal mucosa.