| 用聚乙烯醇为载体固定化枯草杆菌生产α-淀粉酶的研究（Ⅱ）——发酵条件对聚乙烯醇固定化细胞产酶能力的影响及聚乙烯醇固定化细胞的连续发酵 |
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| 引用本文： | 刘智敏 王建华 李泽林. 用聚乙烯醇为载体固定化枯草杆菌生产α-淀粉酶的研究（Ⅱ）——发酵条件对聚乙烯醇固定化细胞产酶能力的影响及聚乙烯醇固定化细胞的连续发酵[J]. 生物医学工程学杂志, 1998, 15(1): 38-40 |
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| 作者姓名： | 刘智敏 王建华 李泽林 |
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| 作者单位： | [1]华西医科大学基础医学院，成都610041 [2]四川联合大学化工学院，成都610065 [3]四川省食品发酵设计院，成都温江611100 |
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| 摘 要： | 研究了一些主要发酵条件对聚乙烯醇(PVA)固定化细胞产酶性能的影响．并在连续进料搅拌槽式反应器中进行了连续发酵试验。结果表明：枯草杆菌经PVA固定化后对pH的适应性有所提高，在连续进料搅拌槽式反应器中连续发酵360h．酶活力保持在170u／ml左右。
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| 关 键 词： | 聚乙烯醇 载体 枯草杆菌 α-淀粉酶 发酵条件 聚乙烯醇 固定化细胞 产酶能力 连续发酵 |
Study on immobilized cells for producing alpha-amylase by using polyving alcohol as the carrier(II): The effect of fermentating conditions on the ability producing alpha-amylase of the cells immobilized with polyving alcohol as the corrier and continuous fermentation of the immobilized cells in CSTR] |
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| Liu Zhiming Wang Jianhua Li Zheling. Study on immobilized cells for producing alpha-amylase by using polyving alcohol as the carrier(II): The effect of fermentating conditions on the ability producing alpha-amylase of the cells immobilized with polyving alcohol as the corrier and continuous fermentation of the immobilized cells in CSTR][J]. Journal of biomedical engineering, 1998, 15(1): 38-40 |
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| Authors: | Liu Zhiming Wang Jianhua Li Zheling |
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| Affiliation: | College of Basic Medicine, West China University of Medical Sciences, Chengdu 610041. |
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| Abstract: | The effects of fermentating conditions on the ability of immobilized cells with PVA as carrier for producing alpha-amylase were studied. The continuous fermentation with the immobilized cells were tested in continuous flow stirred tank reactor (CSTR). The results showed that the adaptability of the immobilized Bacillus substilis to pH increased after immobilization. In CSTR, the immobilized cells can be fermentated continuously for 360 hrs and the activity of alpha-amylase can be kept on the level of about 170 u/ml. |
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