解脲脲原体感染的检测与体外耐药性分析

目的探讨本地区解脲脲原体(Uu)感染及耐药情况,指导临床合理用药。方法对临床送检的531份标本进行Uu培养、计数、鉴定和药敏试验;对775份标本进行荧光定量PCR(FQ-PCR)法和培养法检测Uu。结果FQ-PCR法检出Uu阳性339例,阳性率43.74%。培养法检出Uu206例,阳性率38.79%。Uu对9种抗菌药物的耐药率最高为环丙沙星(CIP)80.10%,最低为交沙霉素(JOS)0.00%,敏感率最高为原始霉素(PRI)98.54%。结论Uu用两种方法检测均可有效检出。培养法能提供药敏结果,更有利于临床治疗。药敏结果提示本地区Uu感染经验用药可选择交沙霉素和强力霉素。

Research on Ureaplasma urealyticum Infections Status and It's Drug-resistance

Objective: To analyze current Ureaplasma urealyticum infection status and its drug-resistance conditions among both urban and sub-urban areas in Yichang; To provide clinical guidelines on administration of antibiotics against diseases caused by Ureaplasma urealyticum infection.Method: The 531 submitted clinical specimens were cultured, enumerated, identificated and antimicrobial sensitivity tested in vitro; 775 submitted specimens were tested by fluorescence qantitative polymerase chain reaction(FQ-PCR).Results: We got 339 Uu (positive rate:43.74%) by FQ-PCR method and 206 Uu (positive rate:38.79%) by cultural method . Among the nine drugs used in our test, ciprofloxacin(CIP) has the highest drug-resistance rate of 80.10% and josamycin(JOS)has the lowest drug-resistance rate of 0.0%. Our tests also showed that pristinamycin(PRI) had the highest drug-susceptivity rate of 98.54% by cultural method.Conclusion: Ureaplasma urealyticum could be identified by both PCR(FQ-PCR) and cultural methods. Cultural method is preferred for clinical practice because it can also provide drug susceptivity results which could be better applied for clinical treatment purpose. Our test showed that josamycin and doxycycline were useful drug.