Industrial kinetic resolution of d,l-pantolactone by an immobilized whole-cell biocatalyst |
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| Authors: | Qiu-Hua Zhang Liu Yang Yi-Bin Tang Liu-Nv Huang Wen-Fang Luo |
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| Institution: | Brother Research Center, Jiangxi Brother Pharmaceutical Co., Ltd, Jiujiang 332700 China, |
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| Abstract: | Immobilized whole-cells of Pichia pastoris harboring recombinant d-lactonase were entrapped in calcium alginate gels and used as an efficient biocatalyst for catalytic kinetic resolution of d,l-pantolactone. The immobilized whole-cell biocatalyst exhibited good catalytic stability, which was applied for stereospecific hydrolysis of d-pantolactone for up to 56 repeated batch reactions without obvious loss in the catalytic activity and enantioselectivity.Immobilized whole-cells of Pichia pastoris harboring recombinant d-lactonase were entrapped in calcium alginate gels and used as an efficient biocatalyst for catalytic kinetic resolution of d,l-pantolactone. |
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