| 高效毛细管电泳法测定苦参中生物碱的含量 |
| |
| 引用本文： | 李顺祥,王实强.高效毛细管电泳法测定苦参中生物碱的含量[J].中国中药杂志,1999,24(2):100-102. |
| |
| 作者姓名： | 李顺祥 王实强 |
| |
| 作者单位： | 湖南省中医药研究院中药研究所 长沙 410013 |
| |
| 摘 要： | 目的：建立苦参中生物碱含量测定新方法。方法：采用高效毛细管电泳法测定。结果：苦参中生物碱能较好分离，加样回收率苦参碱为９７．４７％，氧化苦参碱为９７．６７％。结论：该方法简便、准确、重现性好，可作为苦参中生物碱的质量控制方法。
|
| 关 键 词： | 高效毛细管电泳 苦参 苦参碱 氧化苦参碱 |
| 收稿时间： | 3/6/1998 12:00:00 AM |
Determination of Alkaloids in Radix Sophorae Flavescentis by High Performance Capillary Electrophoresis |
| |
| Li Shunxiang ; Wang Shiqiang.Determination of Alkaloids in Radix Sophorae Flavescentis by High Performance Capillary Electrophoresis[J].China Journal of Chinese Materia Medica,1999,24(2):100-102. |
| |
| Authors: | Li Shunxiang ; Wang Shiqiang |
| |
| Institution: | Hunan Academy of Traditional Chinese Medicine and Materia Medica, Changsha 410013. |
| |
| Abstract: | OBJECTIVE: To develop a simple and rapid method for the determination of alkaloids in Radix Sophorae Flavescentis (RSF). METHOD: HPCE buffer: 20 mmol.L-1 phosphates, pH 7.0; V = 20 kV, leff = 50 cm, L = 64.5 cm, i.d. = 50 microns, lambda = 201 nm, T = 25 degrees C, sample size = 10 kPa.s. RESULT: Matrine(I) and oxymatrine(II) in RSF were completely separated with recoveries of 97.47% and 97.67% respectively. CONCLUSION: Simple, accurate and reproducible, this method can be used for the quality control of Chinese herbal medicines. |
| |
| Keywords: | high performance capillary electrophoresis Radix Sophorae Flavescentis matrine oxymatrine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
