益寿调脂片对衰老模型小鼠自由基代谢作用的实验研究

目的：观察益寿调脂片对衰老模型小鼠自由基代谢调整作用。方法：利用５％Ｄ－半乳糖皮下注射４０天所致亚急性衰老模型小鼠,造模同时分别给予１％淀粉溶液（空白对照组,衰老模型组）,益寿调脂片溶液（低剂抗衰组,高剂抗衰组）及绞股蓝总甙片（阳性抗衰组）４０天后,观察自由基代谢指标如血清丙二醛（ＭＤＡ）,超氧化物歧化酶（ＳＯＤ）,谷胱甘肽过氧化酶（ＧＳＨ－Ｐｘ）及大脑Ｂ型单胺氧化酶（ＭＡＯ－Ｂ）,Ｎａ＾＋－Ｋ＾

Experimental Study of Yishou Tiaozhi Tablet on Free Radical Metabolism in Aging Model Mice

OBJECTIVE: To observe the effect of Yishou Tiaozhi tablet (YSTZT) on the metabolism of free radical in mice of aging model. METHODS: Using the aging model induced by hypodermic injection of 5% D-Galactose in mice for forty days. The content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) in serum, activity of monoamine oxidase B (MAO-B), Na(+)-K(+)-ATPase in brain were investigated after the treatment. RESULTS: In comparing to the aging model group, it showed that YSTZT could obviously inhibit the increase of level of MDA in serum and the activities of MAO-B in brain; and enhance the activities of SOD, GSH-Px in serum, and the activities of Na(+)-K(+)-ATPase in brain. Furthermore, YSTZT could obviously elevate the tolerance to fatigue and hypoxia in mice. CONCLUSIONS: YSTZT can effectively improve the free radical metabolism of senile body, it is an ideal and effective medicine in preventing aging, which is also one of the experimental basis in proving the theory of "Spleen and Kidney deficiency complicated with stasis and phlegm cause aging".