| 芦笋有效成分熊果酸诱导HL-60细胞凋亡的实验研究 |
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| 引用本文： | 黄镜,孙燕,陆士新,苏涛,焦顺昌.芦笋有效成分熊果酸诱导HL-60细胞凋亡的实验研究[J].中国中西医结合杂志,1999,19(5):296-298. |
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| 作者姓名： | 黄镜 孙燕 陆士新 苏涛 焦顺昌 |
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| 作者单位： | 中国医学科学院中国协和医科大学肿瘤研究所肿瘤医院 |
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| 基金项目： | “八五”国家医学科技攻关项目 |
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| 摘 要： | 目的：探讨芦笋有效成分熊果酸对ＨＬ－６０细胞增殖、凋亡的影响。方法：Ｅ和ＭＴＴ法、ＤＮＡ凝胶电泳、形态学方法等测定熊果酸对ＨＨＬ－６０细胞增殖、凋亡的影响。结果：熊果酸抑制ＨＬ－６０细胞增殖，对ＨＬ－６０细胞的半数生长的抑制剂量（ＩＣ５０）为８．２６μｍｏｌ／Ｌ，并能导其发生凋亡结论：熊果酸通过抑制ＨＬ－６０细胞增殖和诱导其凋亡发挥抗肿瘤作用。
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| 关 键 词： | 熊果酸 HL－60细胞 凋亡 中药 药理 |
Experimental Study on Apoptosis Induced by Ursolic Acid Isolated from Asparagus in HL 60 Cells |
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| Authors: | Huang Jing Sun Yan Lu Shixin Cancer Hospital and Institute Peking Union Medical University Chinese Academy of Medical Sciences Beijing |
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| Abstract: | OBJECTIVE: To study the inhibitory effect of ursolic acid isolated from Asparagus on the proliferation of HL-60 cells. METHODS: Effects of ursolic acid on the growth and apoptosis were evaluated by MTT assay, DNA gel electrophoresis and morphology observation respectively. RESULTS: The IC50 value of ursolic acid for HL-60 cells was found to be 8.26 mumol/L and 10-50 mumol/L of ursolic acid could induce apoptosis of HL-60 cells expressed to ursolic acid for 1 day. CONCLUSION: Ursolic acid can markedly inhibit HL-60 cells as well as induction of cells apoptosis. |
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| Keywords: | ursolic acid HL 60 cells apoptosis |
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