| 高效液相色谱法测定何首乌中二苯乙烯甙的含量及其稳定性考察 |
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| 引用本文： | 张纯,杨少麟,袁海龙,郭澄,邵元福. 高效液相色谱法测定何首乌中二苯乙烯甙的含量及其稳定性考察[J]. 中国中药杂志, 1999, 24(6): 357-359 |
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| 作者姓名： | 张纯 杨少麟 袁海龙 郭澄 邵元福 |
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| 作者单位： | 第二军医大学长征医院 上海 200003 |
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| 摘 要： | 目的：建立测定何首乌及其制剂中二苯乙烯甙含量的高效液相色谱法，同时考察二苯乙烯甙的稳定性。方法：二苯乙烯甙测定以ＥｃｏｎｏｓｐｈｅｒｅＣ８５μｍ（２５０ｍｍ×４．６ｍｍ）为固定相，甲醇-水（３０∶７０）为流动相，紫外检测波长为３１０ｎｍ。稳定性考察采用高温实验和酸解实验。结果：进样量在２．２４×１０－２～１．１２×１０－１μｇ范围内线性关系良好，ｒ＝０．９９９８，加样回收率为９９．９６％，ＲＳＤ为１．５４％（ｎ＝６）。结论：本法灵敏、准确、快速；二苯乙烯甙在水溶液中较稳定，但高温（８０℃）对其有一定影响，在酸性溶液中很不稳定。
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| 关 键 词： | 何首乌 二苯乙烯甙 含量测定 稳定性试验 |
| 收稿时间： | 1998-07-01 |
Determination of Stilbene in Radix Polygoni Multiflori by HPLC and Its Stability Study |
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| Zhang Chun;Yang Shaolin;Yuan Hailong;Guo Cheng ; Shao Yuanfu. Determination of Stilbene in Radix Polygoni Multiflori by HPLC and Its Stability Study[J]. China Journal of Chinese Materia Medica, 1999, 24(6): 357-359 |
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| Authors: | Zhang Chun Yang Shaolin Yuan Hailong Guo Cheng Shao Yuanfu |
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| Affiliation: | Changzheng Hospital, Second Military Medical University, Shanghai 200003. |
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| Abstract: | Objective: To develop a HPLC method for determining stilbene in Radix Polygoni Multiflori and studying its stability. Method: HPLC conditions:An Econosphere C18 column,a mixture of methanolwater(3070) as mobile phase,and UV detection at 310nm.In addition,the stability of stilbene was studied at high temperature and in acidic solution. Result: The linear range of calibration curve was 2.2410-21.1210-1g(n=6)with a correlation coefficient of 0.99998,the average recovery with RSD being 99.96%1.54%. Conclusion:The method is sensitive,accurate and speedy.Stilbene in aqueous solution is stable,but high temperature(80) tends to affect the stabilty.In acidic solution no stability can be found. |
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| Keywords: | Polygonum multiflorum stilbene quantitative determination stability study |
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