人参总皂甙增加白血病细胞对化疗药物的敏感性 |
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引用本文: | 高瑞兰,金锦梅,牛泱平,谢桂丽,王文涛,马逢顺.人参总皂甙增加白血病细胞对化疗药物的敏感性[J].中国中西医结合杂志,1999,0(1):17-19. |
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作者姓名: | 高瑞兰 金锦梅 牛泱平 谢桂丽 王文涛 马逢顺 |
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作者单位: | 浙江中医学院附属医院 |
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摘 要: | 目的:探讨人参总皂甙(TSPG)对急性髓细胞白血病(AML)化疗药物敏感性的作用。方法:采用白血病祖细胞集落形成(CFU-AML)药敏试验法。选用4种化疗药物:高三尖杉酯碱(HHr)、阿糖胞苷(Ara)、阿霉素(Adr)和足叶乙甙(Vp-16)。结果:TSPG刺激CFU-AML在体外增殖,集落数提高37.98%,使化疗药物对CFU-AML的抑制率从原先的30.4% ̄47.4%分别提高到51.2% ̄
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关 键 词: | 人参总皂甙 CFU-AML 药敏试验 白血病 治疗 |
Potentiated Effects of Total Saponins of Panax Ginseng on Inhibition of Leukemic Cells by Cytotoxic Drugs |
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Authors: | Gao Ruilan Jin Jinmei Niu Yangping Affiliated Hospital Zhejiang College of Traditional Chinese Medicine Hangzhou |
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Abstract: | OBJECTIVE: To investigate the potentiated effects of total saponins of Panax Ginseng (TSPG) on inhibition of leukemic progenitor cells by cytotoxic drugs in acute myelocytic leukemia. METHODS: Using bone marrow culture of colony forming unite-acute myeloid leukemia (CFU-AML) method, the sensitivity of leukemic cells obtained from 18 patients to homoharringtonin (HHr), cytarabine (Ara), adriamycin (Adr) and etoposide (VP-16) were detected separately. RESULTS: TSPG alone (20 micrograms/ml) could stimulate proliferation of CFU-AML obviously, and increase the colony numbers by 37.98% over the non-TSPG control (P < 0.01). In the presence of TSPG, the inhibition rates of CFU-AML of HHr, Ara, Adr and VP-16 were 51.2%-62.0% respectively, which were significantly higher than 30.4%-47.4% of non-TSPG control (all P < 0.01). In the combination of TSPG with cytotoxic drugs, the leukemic progenitor cells became more sensitive to cytotoxic drugs, CFU-AML colony numbers at 1.84-2.23 fold as more as those of non-TSPG control were inhibited by HHr, Ara, Adr and VP-16. Sensitivity test of 17 among 72 drugs reversed from resistant (suppression rate less than 30%) to sensitive (suppression rate more than 30%) by TSPG. CONCLUSIONS: TSPG could drive non-cycling leukemic progenitors to enter cell cycle, and thereby enhance their susceptibility to cytotoxic drugs. |
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Keywords: | total saponins of Panax Ginseng colony forming unite acute myeloid leukemia drug sensitivity test |
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