脑脊液外泌体miR-146a与人脑胶质瘤术后复发的相关性

目的 探讨脑脊液外泌体miR-146a与人脑胶质瘤术后复发的相关性.方法 收集2016年5月～2017年5月经术后病理证实的70例脑胶质瘤(低级别胶质瘤31例,高级别胶质瘤39例)和25例颅脑损伤(对照组)的脑脊液,采用PCR检测脑脊液外泌体miR-146a表达水平.胶质瘤病人术后随访1～36个月,记录术后复发情况.结...

Correlation between cerebrospinal fluid level of exosomal miR-146a and postoperative recurrence of glioma

Objective To explore the correlation between the cerebrospinal fluid(CSF)level of exosomal miR-146a and the postoperative recurrence of glioma.Methods The levels of exosomal miR-146a were detected in the CSF obtained from 70 glioma patients(31 low-grade and 39 high-grade gliomas)who underwent surgery from May 2016 to May 2017 and from 25 patients with traumatic brain injury(control group)using the PCR.All the glioma patients were followed up from 1 to 36 months.Results The relative expression level of CSF exosomal miR-146a of glioma group was significantly lower than that of control group(P<0.001).The relative expression level of CSF exosomal miR-146a of high-grade gliomas was significantly lower than that of low-grade gliomas(P<0.05).During the follow-up,19 patients(27.14%)had the postoperative recurrence of tumors.Multivariate logistic regression analysis showed down-expression of CSF exosomal miR-146a was an independent risk factor for the postoperative recurrence of glioma patients(P<0.05).ROC curve showed that:the area under curve of the expression level of CSF exosomal miR-146a to predict the postoperative recurrence of glioma patients was 0.853(95%CI 0.762~0.944);the best cut-off value was 0.445,the specificity and sensitivity were 90.2%and 63.2%,respectively;the Youden index was 0.534.Conclusions The expression of CSF miR-146a of patients with glioma is generally down-regulated,and the higher the pathological grade of glioma,the more obvious the decrease of the level of CSF miR-146a.The expression level of CSF miR-146a has certain value for predicting the postoperative recurrence of glioma patients.