脑胶质瘤CHSY1的表达变化及临床意义的生信分析

目的 探讨硫酸软骨素合酶1（CHSY1）在脑胶质瘤中的表达及临床意义。方法 从Gliovis下载CHSY1在TCGA、CGGA和Rembrandt数据库中的基因表达数据和对应的临床数据，将CHSY1表达量由低到高排序，前50%为低表达组，后50%为高表达组。利用TIMER在线网站分析CHSY1在脑胶质瘤中的表达与免疫浸润的相关性。结果 胶质瘤组织CHSY1表达水平明显高于正常脑组织（P<0.05），且与胶质瘤病理分级呈正相关（P<0.05）。多因素Cox回归分析显示CHSY1高表达是胶质瘤病人生存预后不良的独立危险因素（P<0.05），CHSY1高表达组中位生存期较低表达组明显缩短（P<0.05）。MGMT启动子甲基化组胶质母细胞瘤病人中，CHSY1高表达组中位生存期较低表达组明显缩短（P<0.05）。CHSY1高表达组胶质瘤病人化疗后中位生存期较低表达组明显缩短（P<0.05）。TIMER分析发现，胶质母细胞瘤组织CHST1表达水平与树突状细胞的浸润程度呈正相关，低级别胶质瘤组织CHST1表达水平与B细胞、CD8+ T细胞、嗜中性粒细胞、巨噬细胞和树突状细胞的浸润程度成正比。结论 脑胶质瘤CHSY1呈高表达，与胶质瘤的恶性程度与免疫浸润呈正相关。CHSY1可作为胶质瘤预后和对化疗反应的预测因子。

Bioinformatics analysis of CHSY1 expression in brain glioma tissues and its clinical significance

Objective To investigate the expression of chondroitin sulfate synthase 1 (CHSY1) in glioma tissues and its clinical significance. Methods The CHSY1 gene expression data in glioma tissues and the corresponding clinical data of glioma patients in The Cancer Genome Atlas (TCGA), Chinese Glioma Genome Atlas (CGGA), Rembrandt database were downloaded from Gliovis online website. According to the median expression level of CHSY1, the glioma patients were divided into low expression group and high expression group. The correlation between CHSY1 expression and immune infiltration in glioma tissues was analyzed by the online TMER website. Results The expression level of CHSY1 in glioma tissues was significantly higher than that in normal brain tissues (P<0.05), and the expression level of CHSY1 in glioma tissues was positively correlated with the pathological grade of glioma (P<0.05). Multivariate Cox regression analysis showed that high CHSY1 expression was an independent risk factor for poor survival prognosis in the patients with glioma (P<0.05), and the median survival time of high expression group was significantly shorter than that of lower expression group (P<0.05). Of GBM patients with MGMT promoter methylation, the median survival time of high expression group was significantly shorter than that of low expression group (P<0.05). The median survival time of high expression group after chemotherapy was significantly shorter than that of low expression group (P<0.05). TIMER analysis found that the expression level of CHST1 in GBM tissues was positively correlated with the infiltration degree of dendritic cells, and the expression level of CHST1 in LGG tissues was correlated with the infiltration degree of B cells, CD8+ T cells, neutrophils, macrophages and dendritic cells. Conclusions CHSY1 is highly expressed in brain gliomas, which is positively correlated with the malignancy of gliomas and immune infiltration. CHSY1 can be used as a predictor of the prognosis of glioma patients and the response to chemotherapy.