血浆外泌体miR-212、miR-132与颅脑损伤严重程度和Rotterdam CT评分的关系

目的 探讨血浆外泌体miR-212、miR-132水平与颅脑损伤（TBI）严重程度和Rotterdam CT评分的关系。方法 前瞻性收集2019年3月~2020年10月收治的TBI共76例，另选择我院体检的健康成年人38例为对照。提取血浆外泌体并采用实时荧光定量PCR法检测miR-212、miR-132表达水平。结果 76例TBI中，轻型19例，中型35例，重型22例。TBI血浆外泌体miR-212、miR-132水平均明显低于对照组（P<0.05）。与轻型TBI比较，中、重型TBI组血浆外泌体miR-132水平明显降低（P<0.05）；与中型TBI比较，重型TBI血浆外泌体miR-212、miR-132水平明显降低（P<0.05）。血浆外泌体miR-212（（r=0.396）、miR-132（r=0.716）水平与入院GCS评分呈明显正相关（P<0.001）。血浆外泌体miR-212水平（r=-0.375）和miR-132水平（r=-0.613）与入院Rotterdam CT评分呈明显负相关（P<0.05）。结论 TBI病人血浆外泌体miR-132、miR-212水平明显下降，并且随着TBI损伤程度加重和入院Rotterdam CT评分增加而明显降低。这提示血浆miR-132、miR-212可能是TBI潜在的生物标记物。

Relationship of plasma exosomal levels of miR-212 and miR-132 to severity and Rotterdam CT score of patents with traumatic brain injury

Objective To investigate the relationship of plasma exosomal levels of miR-212 and miR-132 to the severity and Rotterdam CT score of patients with traumatic brain injury (TBI). Methods A total of 76 TBI patients were enrolled prospectively from March 2019 to October 2020 and 38 healthy volunteers were recruited as the control. The plasma exosomal levels of miR-212 and miR-132 were detected using real-time fluorescent quantitative PCR. Results Of 76 TBI patients, 19 suffered from mild TBI, 35 moderate and 22 severe. The plasma exosomal levels of miR-212 and miR-132 in the TBI group significantly decreased compared to those in control group (P<0.05). As the severity of TBI increased, the plasma exosomal levels of miR-212 and miR-132 significantly decreased(P<0.05). The plasma exosomal levels of miR-212 (r=0.396) and miR-132 (r=0.716) were positively significantly correlated with the GCS score on admittion (P<0.05). The plasma exosomal levels of miR-212 (r=-0.375) and miR-132 (r=-0.613) were negatively significantly correlated with the Rotterdam CT score on admittion (P<0.05). Conclusions The plasma exosomal levels of miR-132 and miR-212 in TBI patients significantly decrease, and they decrease significantly as the severity of TBI increase and the Rotterdam CT score increase. Our results suggest that the plasma exosomal levels of miR-132 and miR-212 may be potential biomarkers of TBI.