显微镜下多血管炎患者外周血髓样树突状细胞和调节性T细胞的表达及意义

目的 探讨显微镜下多血管炎(MPA)患者外周血髓样树突状细胞(mDC)、调节性T细胞(Treg)水平与伯明翰系统性血管炎活动评分(BVAS)、肾组织病理损伤之间的关系.方法 选择27例活跃期MPA患者(MPA活跃组)和18例缓解期MPA患者(缓解组),并以性别、年龄相匹配的健康志愿者15例为对照组,采用流式细胞技术三色...

Expression and significance of myeloid dendritic cells and regulatory T cells in peripheral blood of patients with microscopic polyangiitis

Objective To investigate the correlation between the levels of myeloid dendritic cells(mDC),and regulatory T cells(Treg)in peripheral blood of patients with microscopic polyangiitis(MPA)and the Birmingham vasculitis activity score(BVAS)as well as the pathological damage of kidney tissue.Methods Twenty-seven patients with active MPA(MPA active group)and 18 patients with MPA in remission(MPA remission group)were selected,and 15 healthy volunteers with matching gender and age were selected as a control group.The percentage of mDC in lymphocytes and the percentage of Treg cells in CD4+cells in peripheral blood were detected by using flow cytometry three-color analysis.The mDC was defined as Lin1^-HLA-DR⁺CD11c⁺,and Treg was defined as CD4+CD25highCD127lowTreg cells.According to WHO pathological diagnosis criteria,24 MPA patients with renal biopsy were divided into focal type group(n=12),crescent type group(n=5)and mixed type group(n=7).Results The percentage of mDC in lymphocytes and the percentage of CD4⁺CD25^highCD127^lowTreg in CD4+cells in peripheral blood in the MPA active group were lower than those in the MPA remission group and the control group(P<0.05).The proportions of mDC,and CD4⁺CD25^highCD127^lowTreg cells were negatively correlated with BVAS score(P<0.05).The percentage of mDC in lymphocytes and the percentage of CD4⁺CD25^highCD127^lowTreg cells in CD4⁺cells in the crescentic group were lower than those in the focal group and the mixed group(P<0.05).Conclusion The decreased levels of mDC and CD4⁺CD25^highCD127^lowTreg cells in peripheral blood are related to disease activity and acute pathological injury of kidney tissue in MPA patients.