慢性阻塞性肺疾病患者痰液白细胞介素8,6和肿瘤坏死因？…

目的：探讨３种细胞因子和炎症细胞在慢性阻塞性肺疾病（ＣＯＰＤ）发病机制中的作用。方法收集２０例稳定期ＣＯＰＤ患者、１０名健康吸烟者（ＨＳ）和１０名健康不吸烟者（ＨＮＳ）的痰液。对痰液进行炎症细胞计数与分类检查，并用放射免疫法测定痰液中白细胞介素８（ＩＬ－８）、白细胞介素６（ＩＬ－６）与肿瘤坏死因子α（ＴＮＦ－α）水平。结果ＣＯＰＤ组痰液ＩＬ－８、ＴＮＦ－α水平及中性粒细胞分类（ＰＭＮ）明显高于ＨＳ

Detection and significance of interleukin-8

OBJECTIVE: To evaluate the role of cytokines in the pathogenesis of COPD. METHODS: Induced sputum samples were obtained from 20 patients with stable COPD, 10 healthy smokers (HS) and 10 healthy nonsmokers (HNS). Total and differential cell counts were examined, and sputum interleukin (IL)-8, tumor necrosis factor (TNF)-alpha, IL-6 level were measured by radioimmunoassay technique. RESULTS: Sputum IL-8, TNF-alpha level and neutrophil percentages (PMN) in the COPD group were significantly higher than those in the HS group and the HNS group (P < 0.01). Compared with the HNS group, there was a significant increase in the PMN and the level of IL-8 in the HS group (P < 0.05). The sputum level of IL-6 did not differ significantly among three groups (P > 0.05). In the COPD group, the sputum level of IL-8 was correlated positively with the level of TNF-alpha and the PMN (r's = 0.5999, 0.6737). In the HS group the sputum level of IL-8 was also correlated positively with the PMN (rs = 0.8424). The sputum IL-8, TNF-alpha levels and the PMN were all correlated negatively with FEV1% and FEV1/FVC in the COPD group (r's = -0.6453, -0.7341, -0.7341, -0.7624, -0.5262, -0.7353). CONCLUSIONS: IL-8, TNF-alpha and neutrophil are involved in airway inflammation which results in airflow obstruction, thus playing an important role in the pathogenetic process of COPD.