| 对测定饱和脂肪酸含量的高效液相色谱－化学发光系统的探讨 |
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| 引用本文： | 段更利,中岛宪一郎,秋山修三,黑田直敬.对测定饱和脂肪酸含量的高效液相色谱－化学发光系统的探讨[J].中国药学杂志,1996(11). |
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| 作者姓名： | 段更利 中岛宪一郎 秋山修三 黑田直敬 |
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| 作者单位： | 上海医科大学药物分析教研室，日本长崎８５２长崎大学药学部 |
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| 摘 要： | 目的：探讨测定脂肪酸含量的高效液相色谱－化学发光系统及其最佳化的条件。方法：将用荧光试剂衍生化后的脂肪酸用高效液相色谱－化学发光检测法分析。结果：在ＴＣＰＯ－Ｈ２Ｏ２－咪唑化学发光系统的最佳化条件下，测得脂肪酸Ｃ１６，Ｃ１８，Ｃ２０的检测限分别为１２，１８，４４ｆｍｏｌ（１０－１５ｍｏｌ）。结论：建立的测定脂肪酸含量的高效液相色谱－化学发光检测法的灵敏度极高，已达ｆｍｏｌ级
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| 关 键 词： | 脂肪酸 高效液相色谱法 化学发光检测 |
The systems of high performance liquid chromatography/chemiluminescence for determination of the saturated fatty acids |
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| Duan Gengli.The systems of high performance liquid chromatography/chemiluminescence for determination of the saturated fatty acids[J].Chinese Pharmaceutical Journal,1996(11). |
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| Authors: | Duan Gengli |
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| Abstract: | OBJECTIVE:To investigate the HPLC/chemiluminescence system and the optimum conditions for determination of fatty acids.METHOD:Derived by the fluorescent reagent HCPI,the fatty acids were determined by a HPLC/chemiluminescence detection method.RESULTS:With the optimum conditions of the TCPO H 2O 2 imidazole chemiluminescence system,the detection limits of FA C 16 ,C 18 and C 20 were 12,18 and 44 f mol,respectively.CONCLUSION:The sensitivity of fatty acids determined by HPLC/chemiluminescence detection method established was very high and it had been f mol grade. |
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| Keywords: | fatty acids chemiluminescence detection high performance liquid chromatography |
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