CD4+C25+Treg细胞与喉鳞状细胞癌的相关性研究

［摘要］目的：研究CD4+CD25+Treg细胞与喉鳞状细胞癌的相关关系。方法：收集2005年7月至2005年12月山东大学齐鲁医院耳鼻咽喉科手术治疗的30例喉鳞状细胞癌患者和同期13例健康献血者的外周血，应用流式细胞仪检测外周血中CD4+CD25+Treg细胞的百分比，用ELISA法检测外周血中TGF β，IL 10，IFN γ的含量。结果：喉鳞状细胞癌患者与正常对照组外周血中CD4+CD25+Treg细胞的百分比分别为（8.72±1.54）%和（4.64±0.93）%，喉鳞状细胞癌组高于正常对照组(P＜0.05)，但与性别、年龄、临床分期、淋巴结转移无关。喉鳞状细胞癌患者外周血中TGF β，IL 10含量分别为(10.96±1.19)?pg/ml和(15.87±2.17)?pg/ml，高于正常对照组的(6.77±0.72)?pg/ml,(1.8±2.5)?pg/ml (P＜0.05)；但IFN γ的含量为(16.67±8.39)?pg/ml，低于正常对照组的(21.98±9.64)?pg/ml(P＜0.05)。结论：CD4+CD25+Treg细胞在喉癌患者外周血中大量增加,下调T细胞介导的肿瘤免疫。

Relationship between CD4 + CD25 + Treg and laryngeal carcinoma

ABSTRACT］Objective: To evaluate the correlation between CD4+CD25+Treg cell and laryngeal carcinoma. Methods: Peripheral blood was assembled from 30 patients with laryngeal carcinoma and 13 healthy persons. The flow cytometry was used to detect the percent of CD4+CD25+Treg cell, and the enzyme labeled immunosorbent assay was used to detect the content of TGF β，IL 10 and IFN γ. Results: The percent of CD4+CD25+Treg cell in peripheral blood of laryngeal carcinoma was higher than that of healthy persons ［(8.72±1.54)% vs (4.64±0.93)%］ (P＜0.05), and the content of TGF β，IL 10 in peripheral blood of laryngeal carcinoma was higher than that of health persons［(10.96±1.19)?pg/ml vs (6.77±0.72)?pg/ml, and (15.87±2.17)?pg/ml vs (1.8±2.5)?pg/ml, respectively］(P＜0.05), whereas the content of IFN γ in the laryngeal carcinoma was lower than that of the healthy persons 〔(16.67±8.39)?pg/ml vs (21.98 ±9.64)?pg/ml〕(P＜0.05). Conclusion: CD4+CD25+Treg cell increases in the peripheral blood of laryngeal carcinoma and down regulates the tumor immunity mediated by T cell.