TIA患者血清IL-6、IL-18水平与颈动脉内中膜厚度的关系

目的 通过分析颈动脉内中膜厚度(IMT)与血清细胞因子白介素-6(IL-6)、白介素-18(IL-18)水平的关系,探讨IMT增厚的发生机制.方法 选择66例短暂性脑缺血发作(TIA)患者(TIA组)作为研究对象,另选择40名健康者作为正常对照组.分别测量其颈动脉IMT值,并分为IMT＜1.0mm亚组和IMT≥1.0mm亚组.利用液相平衡竞争放射免疫分析法(RIA)和酶联免疫吸附法(ELISA)分别测定了2组的血清IL-6和IL-18水平,并对IMT与IL-6,IL-18的关系进行分析.结果 TIA组所有IMT≥1.0mm患者血清IL-6、IL-18含量均比正常对照组升高,差异有统计学意义(P＜0.05,P＜0.01); TIA组IMT≥1.0mm亚组患者血清IL-6、IL-18含量比IMT＜1.0 mm亚组患者升高,差异有统计学意义(P＜0.05).经Pearson相关分析,TIA组IMT≥1.0mm亚组患者IMT与血清IL-18含量呈明显正相关(r=0.551,P＜0.01)；TIA组、脑梗塞组IMT≥1.0mm亚组患者IMT与血清IL-6含量无相关性(r=0.303,P=0.058).结论 TIA组IMT增厚的患者血清IL-6、IL-18的水平是升高的.IMT的增厚与血清IL-18含量呈明显正相关,与血清IL-6含量无相关性.IL-6、IL-18可能参与了AS的形成过程.

Relationships between Carotid Artery lntima-media Thickness and Serum Interleukin-6 and Interleukin-18 on TIA patients

Objective To investigate the mechanisms of carotid artery intima-media thickness （IMT} and the relation- ships between carotid artery IMT and the serum levels of interleukin 6, interleukin 18. Methods Carotid artery IMT values were prospectively measured by color Doppler ultrasound in sixty--six cases of transient ischemia attack （TIA） patients who as the study group, and fort}, heathy volunteers as control group. The study groups were divided into two subgroups according to IMT larger or lower than 1.0 mm. Serum IL-6, IL-18 levels were determined in all patients and volunteers with radioimmunoassay{ RIA）and enzyme-linked immunosorbent assay { ELISA） respec- tively. Results There were significant differences in IL-6 and IL-18 levels between IMT~I. 0 mm subgroups and control group （P~0.05, P%0.01）. The IL-6 and IL-18 levels between IMT~I. 0 mm and IMT%I. 0 mm sub- groups had significant differences （P〈0.05）. The levels of serum IL-18 were positively correlated with IMT values in IMT≥1. 0 mm subgroups（Pearson correlation coefficients r= 0.551, P%0.01）, and the levels of serum IL 6 had no correlation with IMT values in IMT≥1. 0 mm subgroups（Pearson correlation coefficients r=0.303, P= 0. 058）. Conclusion The levels of serum IL-6, IL-18 are increased in TIA patients with IMT thickness increased. Serum IL- 18 level is positively correlated with carotid artery IMT,and no correlation between serum IL-6 level and IMT. IL-6 and IL-18 may involve in the development of AS.