大鼠肝癌肿瘤转移相关基因的表达及不同中医治法作用

目的：观察肿瘤转移相关基因及转移抑制基因在DEN诱发大鼠肝癌组织中的表达及不同治法对其影响。方法：DEN诱发大鼠肝癌，经西药喃氟啶和不同中药治疗，取大鼠肝（含肝癌）组织抽提RNA，Affymetrix Rat 230A GeneChip检测基因表达的差异。结果：肝癌表达增加者55个，减少者8个，不同中西医治法有一定的调节作用。分析：肿瘤转移相关基因及转移抑制基因在肝癌中表达的改变和不同治法作用的差异，其具体调节途径和意义有待进一步研究。

The expression of tumor metastasis related genes in rat''''s hepatocellular carcinoma and effect of several curing method

Objective: To observe the expression of tumor metastasis related genes and effect of several curing method on rat's liver cancer induced by DEN.Method: Rat's HCC was induced by DEN,then treated by Tegafur or 3 different traditional Chinese medicines.RNA extracted from rat's hepatic tissue(including liver cancer),and 10 Affymetrix Rat 230A GeneChips were used to detect the difference of genes expression.Results: In Rat 230A genechip,there were 55 up-expression genes and 8 down-expression genes in liver cancer,and these genes could be regulated partly by different curing method.Analysis: Further research will need to do for investigating the regulating pathway of different curing method and the role of tumor metastasis related genes and metastasis suppressor genes in liver cancer.