ＲｈｏＡ小干扰ＲＮＡ抑制乳腺癌细胞株ＭＣＦ-７及其裸鼠皮下移植瘤的实验研究

目的　观察ＲｈｏＡ小干扰ＲＮＡ（ｓｉＲｈｏＡ）对乳腺癌细胞株ＭＣＦ-７增殖、迁移、周期和凋亡的影响以及对裸鼠移植瘤生长的影响。方法　ｓｉＲｈｏＡＬｉｐｏｆｅｃｔａｍｉｎｅ２０００介导下转染乳腺癌细胞ＭＣＦ-７，转染４８ｈ后，采用Ｗｅｓｔｅｒｎｂｌｏｔ技术检测ＲｈｏＡ蛋白的表达，ＭＴＴ实验检测ｓｉＲｈｏＡ转染细胞的增殖变化，损伤刮擦实验检测ｓｉＲｈｏＡ转染细胞的迁移能力，流式细胞仪检测ｓｉＲｈｏＡ转染细胞的周期和凋亡，裸鼠移植瘤实验检测ｓｉＲｈｏＡ对肿瘤生长的影响。结果　成功转染ｓｉＲｈｏＡ的肿瘤细胞，Ｗｅｓｔｅｒｎｂｌｏｔ显示ＲｈｏＡ蛋白表达在ＭＣＦ-７细胞中明显下降；ｓｉＲｈｏＡ对ＭＣＦ-７细胞的增殖、迁移均有显著的抑制作用并能促进肿瘤细胞凋亡及细胞周期中Ｓ期细胞减少，Ｇ１／Ｇ０期细胞增加；裸鼠移植瘤内重复注射ｓｉＲｈｏＡ后肿瘤生长明显减缓。结论　ｓｉＲｈｏＡ能够明显抑制ＲｈｏＡ基因在乳腺癌细胞ＭＣＦ-７中的表达，部分逆转ＭＣＦ-７的恶性生物学行为并抑制裸鼠移植瘤的生长。

Inhibition of the biological behaviors of MCF-7 cell line and tumor growth of nude mice model by RhoA siRNA

Objective To explore the effect of RhoA siRNA on the biological behaviors of breast cancer cell MCF-7 and the tumor growth of nude mice model.Methods RhoA siRNA was transfected into human breast cancer cell line MCF-7 by Lipofectamine2000.The expression of RhoA in MCF-7 was detected by Western blot after 48 hours of the transfection.The effect of RhoA on the proliferation of MCF-7 was tested by MTT assay.The motility of the transfected cancer cells were assessed by wound-healing assay.The tumor cell cycle a...