Involvement of Spinal Cord Nuclear Factor kB Activation in A Rat Model of Persistent,Postoperative Pain Evoked by Skin/Muscle Incision and Retraction (SMIR) |
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作者姓名: | Su-zhen Fan Xing-guo Hu dun Yan Gong-sheng Zou Yin-ming Zeng |
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作者单位: | [1]Jiangsu Province Key Laboratory of Anesthetic and Analgesic Application Technology, Xuzhou Medical College, Xuzhou, 221002,China [2]Department ofAnaesthesiology, Taoyuan county People's HospitaI,Hunan ,415700, China [3]Department of Anaesthesiology, Xiangtan Center Hospital, Hunan, 411100, China |
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基金项目: | Acknowl from China Department edgement: This work was supported by grants Hunan Provincial Science and Technology (2009SK3018) |
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摘 要: | Background: Persistent postoperative pain is common but the pathogenic mechanisms underlying it are under debate. Recent studies have implicated the activation of nuclear factor kappa B (NF-kB) in spinal for development of neuropathic and inflammatory pain. Aims: The aim of the present study was to investigate whether NF-kB also mediates hyperalgesia in persistent postoperative pain and potentially the pathogenesis of postoperative pain, by measuring NF-kB levels and assessing the anti-allodynic effect of NF-r.B inhibition by pyrrolidine dithiocarbamate (PDTC) in rats after skin/muscle incision and retraction (SMIR) injury. Methods: After rats received a SMIR surgery, mechanical allodynia were determined by von Frey filaments, and NF-kB p65 in the spinal cord was quantified to determine the level NF-KB expression at different time points after SMIR by western blotting. The NF-kB inhibitor PDTC was administered intrathecally at day 1 after surgery and then daily for 7 days to determine the role of NF-KB in postoperative pain, Results: A significant increase in level of NF-kB p65 was observed in the spinal cord from day 1 to day 7 after SMIR, Intrathecal injection of PDTC attenuated SMIR-induced mechanical allodynia from day 3 to day 12. Conclusions: SMIR-induced mechanical allodynia can be partially reversed by the NF-kB inhibitor. Our results suggest that NF-r.B activation in spinal cord plays a role in SMIR-induced mechanical allodyuia in rats. Therefore, NF-kB inhibition may be useful but not sufficient for the therapeutic control of postoperative pain,
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关 键 词: | 麻醉学 手术后 临床护理 PDTC |
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