知母皂甙元对东莨菪碱所致学习记忆障碍和脑胆碱乙酰转移酶活力降低的影响

目的:观察知母活性成分知母皂甙元(ZMS)对学习记忆和脑中胆碱乙酰转移酶(ChAT)活性的影响。方法:腹腔内一次注射东莨菪碱建立小鼠拟痴呆模型,用避暗法及跳台法测定学习记忆能力,用~3H-乙酰辅酶A掺入法测定脑组织ChAT活性。结果:ZMS可使东莨菪碱拟痴呆小鼠在学习记忆实验中的错误次数明显减少,潜伏期显著延长,并能显著增强脑内ChAT活性。结论:ZMS能够提高东莨菪碱拟痴呆小鼠的学习记忆能力,增强脑内ChAT的活性,但是,由于ZMS提高ChAT活性没有明显的量效关系,而且Tacrine也有类似作用,所以很可能不是直接提高ChAT活性,而是通过提高脑M受体密度实现的。

Tne Effects of ZMS on Learning and Memory Ability and BrainCholine Acetyltransferase in Scopolamine-induced Mouse Model

Objective: To observe the effects of ZMS on learning and memory abillity and on brain choline acetyltransferase activity.Methods: A single intraperitoneal injection of scopolamine was used to establish a dementia mouse model.The learning and memory a-bility was detected by step-through and sted-down tests. And the choline acetyltransferase activity in brain was determined by 3 H-acetyl-CoA incorporation analysis. Results: ZMS could significantly reduce wrongness frequences, prolong incubation period of scopolamine-induced dementia mice in step-through and step-down tests (P < 0.01) .ZMS could also significantly improve the activity of brain choline acetyltransferase (ChAT)(P<0.01).Conclusion:ZMS could improve the learning and memory ability and the activity of brain ChAT in scopolamine-induced dementia mice. However,ZMS had no significant dose-response efiect on improving the activity of brain ChAT, and the effect of Tacrine was similar to that of ZMS, it seemed unlikely that ZMS had a direct action on the ChAT. More probably, ZMS exerted its effect on ChAT activity and on learning and memory ability via elevating brain M receptor density.