简易雪旺氏细胞培养方法及用于凋亡实验的研究

目的：寻找一种快速简便的雪旺氏细胞培养方法以期获得研究神经再生的细胞材料并用于细胞凋亡的实验研究。方法：采用两次差速贴壁结合Ara-c法获得雪旺氏细胞，加入过氧化氢并经1％的碘化吡啶（PI）染色观察雪旺氏细胞凋亡的情况。结果；采用该法能获得较纯的雪旺氏细胞，加入过氧化氢后经1％的碘化(PI)杂色雪旺氏细胞有不同程度的凋亡。结论：过氧化氮产生的氧自由基能诱导细胞的凋亡，提示在神经组织的创伤过程中神经胶质细胞的凋亡可能与氧自由基损伤有关。

Study of a simplified method for culturing Schwann cells and its application in apoptosis experiment

Objective To seek a quick and simple protocol in Schwann cells culture and applied to in vivo apoptosis study for further study of nerve regeneration. Methods Schwann cells were acquired with two speedy adesion and Ara-c method, and the condition of apoptosis was examined by adding hydrogen peroxide and 1% PI staining. Results Purified Schwann cells were obtained and apoptosis was found in some extent after staining. Conclusion Hydrogen peroxide can induce Schwann cells apoptosis and suggest neuroglia cells apoptosis may concern with the free oxide duing the nerve tissue injury.