一些化工原料的雌激素样效应

采用雌激素受体阳性乳腺癌细胞株T47D在DMEM培养基 (含 1 0 %小牛血清 )中开放式单层贴壁培养的方法 ,探讨几种常见化工原料壬基酚 (NP)、双酚A(BisA)、邻苯二甲酸酯二丁酯 (DBP)的雌激素效应。于开始实验前将培养细胞用PBS洗涤后改为在无酚红DMEM(含 5 %CDT -FBS)中培养持续 5d ,其目的是耗尽内源性雌激素 ,实验设溶剂对照、雌激素阳性对照及 3种受试物各 4个剂量组 ,采用MTT法、3 H TdR掺入法及流式细胞术对T47D细胞增殖情况进行分析。结果显示NP、BisA和DBP对T47D细胞增殖的影响同雌激素类似 ,可促进T47D细胞增殖和细胞DNA合成 ,并推进G0 G1 期细胞进入S期 ,提高细胞增殖指数 ,随着培养时间 ,延长至 96h ,0 8μmol LNP、32 μmol LBisA和 32 0 μmol LDBP均对T47D细胞有明显促进增殖效果。结果提示这几种化合物具有雌激素效应 ,且其作用效果存在时间 -效应和剂量 -效应关系。由于T47D细胞增殖为雌激素依赖性 ,由此可推测它们有可能通过雌激素受体而发挥其作用的

Estrogenic activity of some environmental chemicals

The study was designed to explore the estrogenic activity of environmental disrupters n 4 noniphenol (NP), bisphenol A (bisA) and dibutylphthalate (DBP)]. Estrogen respective breast cancer cell line T47D was grown in DMEM medium containing 10% bovine serum. Five days before the addition of the test compounds, the cell were washed in phosphate buffered saline, and the medium was substituted with a phenol red free DMEM medium containing 5% dextran charcoal stripped FBS. The respective test compound was added in fresh medium and the control cell received only the vehicle (ethanol). The prilieration of T47D cells was analyzed by MTT assay, 3H TdR incoporation assay and flow sytometer. The results indicated that, compared with the EtOH control cell, the test compounds (n 4 noniphenol, Bisphenol A and dibutylphthalate), like estradiol, markedly enhanced the prolieration of T47D cell and the metaphase of cell division, and the results showed time dependent and dose dependent model. These data showed that the tested chemicals could enhance the prolieration of human vervix cancer cell in vitro. This might hinted that these chemicals possessed estrogenic activity and they might play their estrogen through estrogenic receptor.