| 尾叶香茶菜二萜类化合物F对肝癌细胞株SMMC-7721的影响 |
| |
| 引用本文： | 孙明莉,孙晓娟,张宏宇,孔晓霞,袁兆新,穆桂芳,李扬,王冠军,李洪岩. 尾叶香茶菜二萜类化合物F对肝癌细胞株SMMC-7721的影响[J]. 临床肝胆病杂志, 2006, 22(5): 344-346 |
| |
| 作者姓名： | 孙明莉 孙晓娟 张宏宇 孔晓霞 袁兆新 穆桂芳 李扬 王冠军 李洪岩 |
| |
| 作者单位： | 吉林大学第一医院急救医学科,吉林,长春,130021;吉林大学中日联谊医院妇产科,吉林,长春,130000;吉林大学基础医学院病理生理学教研室,吉林,长春,130021 |
| |
| 基金项目： | 吉林省科技厅医学专项基金(2006-0271) |
| |
| 摘 要： | 观察尾叶香茶菜中提取物二萜类化合物F对人肝癌细胞株SMMC-7721的作用并探讨其机制。分别应用MTT法、AO／EB荧光染色法分析细胞存活率和凋亡情况,利用DCFH-DA荧光分光光度法检测细胞内过氧化物(ROS)的水平。二萜类化合物F抑制SMMC-7721细胞增殖,且呈时间剂量依赖性,二萜类化合物F作用的SMMC-7721细胞内ROS水平明显升高。二萜类化合物F可以启动细胞内氧化应激诱导SMMC-7721细胞坏死或凋亡而抑制肝癌细胞株增殖。
|
| 关 键 词： | 二萜类化合物F 尾叶香茶菜 肝癌细胞株 氧化应激 |
| 文章编号： | 1001-5256（2006）06-0344-03 |
| 收稿时间： | 2005-12-20 |
| 修稿时间： | 2006-01-26 |
Apoptosis in SMMC - 7721 cells induced by diterpenoid F derived from rabdosia excise |
| |
| SUN Ming - li, SUN Xiao -juan, ZHANG Hong -yu,et al.. Apoptosis in SMMC - 7721 cells induced by diterpenoid F derived from rabdosia excise[J]. Chinese Journal of Clinical Hepatology, 2006, 22(5): 344-346 |
| |
| Authors: | SUN Ming - li SUN Xiao -juan ZHANG Hong -yu et al. |
| |
| Affiliation: | 1. Deportment of ICU, the First Clinic Hospital, Jilin University, Chongchun 130021 |
| |
| Abstract: | To determine the apoptosis of human hepatoma carcinoma cell line SMMC -7721 induced by diterpenoid F derived from radbosia excise, and to study its mechanism. The survival and apoptotic rate of SMMC -7721 cells were detected by MTT and AO/EB fluorescent staining respectively. And the intracellular ROS level was analyzed by fluorospectro-photometry. Diterpenoid F could inhibit the proliferation of SMMC -7721 in a dose - and time - dependent manner. The intracellular ROS increases in SMMC -7721 through oxidative stress pathway. |
| |
| Keywords: | diterpenoid F rabdosia excise apoptosis hepatic cell line oxidative stress |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
