微孔药敏检测法检测耐多药结核病 33例耐药分析

目的 探讨MicroDSTTM(微孔药敏检测法)微孔板法检测结核分枝杆菌的耐药情况分析.方法 用MicroDSTTM微孔板法对贵阳市公共卫生救治中心2018年1月至2019年11月期间部分用比例法药敏试验随机筛选的33例耐多药结核病(MDR-TB)阳性菌株进行16种抗结核药药物敏感性检测.结果 异烟肼、利福平耐药率与比例法比较符合率为97.0％(32/33)、93.9％(31/33);对16种药总耐药率33％(174/528);一线总耐药率70.5(93/132)、二线总耐药率20.5(81/396).结论 微孔板法检测的MDR-TB病人筛选率与比例法具有较高符合率、且本地区MDR-TB病人的耐药情况较严重,应加强药物监测,选择有效药物化疗,而MicroDSTTM微孔板法的推广使用有利于MDR-TB诊断和药物筛选.

Analysis of drug resistance in 33 cases of multidrug-resistant tuberculosis by microporous antimicrobial susceptibility testing

Objective To explore the analysis of drug resistance of Mycobacterium tuberculosis detected by MicroDSTTM (micropo-rous drug sensitivity test) microplate method.Methods A total of 33 multi-drug resistant tuberculosis (mDR-TB) positive strains ran-domly selected by proportional method in Guiyang Public Health Treatment Center from January 2018 to November 2019 were selectedfor drug susceptibility detection of 16 anti-tuberculosis drugs by MicroDSTTM microplate method.Results The drug resistance detec-tion rate of isoniazid and rifampicin were 97.0% (32/33) and 93.9% (31/33), respectively. The total drug resistance rate to 16 drugs was33% (174/528), the first-line total drug resistance rate was 70.5 (93/132) and the second-line total drug resistance rate was 20.5 (81/ 396).Conclusion The screening rate of MDR-TB patients detected by microplate method is in high accordance with the proportion method, and the drug resistance of MDR-TB patients in this area is serious, so it is necessary to strengthen drug monitoring and selecteffective drug chemotherapy, while the promotion and use of MicroDSTTM microplate method is conducive to MDR-TB diagnosis and drug screening.