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Anti-miRNA-21增加肝细胞癌Huh 7细胞对吉非替尼的敏感性
引用本文:彭洪清,李光照,向琼. Anti-miRNA-21增加肝细胞癌Huh 7细胞对吉非替尼的敏感性[J]. 中国药科大学学报, 2012, 43(4): 371-375
作者姓名:彭洪清  李光照  向琼
作者单位:娄底市中心医院;娄底市中心医院;南华大学药物药理研究所
摘    要:为了探讨anti-miRNA-21与肝细胞癌Huh 7细胞对吉非替尼敏感性之间的关系,通过MTT法和流式细胞术检测anti-miRNA-21联合吉非替尼对Huh 7细胞增殖和凋亡的影响。用Western blot检测anti-miRNA-21对同源性磷酸酶-张力蛋白(PTEN)表达的影响,以及联合吉非替尼对ERK/Akt信号通路相关蛋白表达的影响。结果显示,anti-miRNA-21联合吉非替尼可显著抑制Huh 7细胞增殖(P<0.05),并促进其凋亡;anti-miRNA-21可以上调Huh 7细胞中PTEN的表达;miRNA-21联合吉非替尼对ERK/Akt信号通路的抑制高于吉非替尼组。结果表明,anti-miRNA-21可以增加肝细胞癌Huh 7细胞对吉非替尼的敏感性。

关 键 词:anti-miRNA-21  吉非替尼  凋亡  肝细胞癌  敏感性

Anti-miRNA-21 increases sensitivity of hepatocellular carcinoma Huh 7 cells to gefitinib
PENG Hong-qin,LI Guang-zhao and XIANG Qiong. Anti-miRNA-21 increases sensitivity of hepatocellular carcinoma Huh 7 cells to gefitinib[J]. Journal of China Pharmaceutical University, 2012, 43(4): 371-375
Authors:PENG Hong-qin  LI Guang-zhao  XIANG Qiong
Affiliation:1Loudi Central Hospital,Loudi 417000;2Institute of Pharmacological Research,University of South China,Hengyang 421001,China
Abstract:In order to investigate the effect of anti-miRNA-21 on the sensitivity of hepatocellular carcinoma cell lines Huh 7 to gefitinib,MTT assay and flow cytometry were used to detected the proliferation and apoptosis of Huh 7 cells transfected by anti-miRNA-21 on the induction of gefitinib.Western blot was used to detect the expression of phosphatase and tensin homolog(PTEN) and relative protein of ERK/Akt pathway.The results showed that anti-miRNA-21 combining with gefitinib inhibit the proliferation of Huh 7 cells significantly(P<0.05);and inhibited the expression of ERK/Akt pathway related protein more efficiently.Anti-miRNA-21 could upregulate the expression of PTEN in Huh 7 cells.In conclusion,anti-miRNA-21 increased the sensitivity of hepatocellular carcinoma Huh 7 cells to gefitinib.
Keywords:anti-miRNA-21  gefitinib  apoptosis  hepatocellular carcinoma  sensitivity
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