三氧化二砷诱导乳腺癌细胞凋亡的早期事件——凋亡性容量下降

目的探讨凋亡性容量下降(apoptotic volume decrease,AVD)是否是三氧化二砷(arsenic trioxide,As2O3)诱导乳腺癌MCF-7细胞凋亡的早期事件。方法噻唑兰(MTT)法检测As2O3对MCF-7细胞存活率的影响;流式细胞技术检测As2O3能否引起MCF-7细胞凋亡;时滞显微摄影技术检测细胞容量;Caspase-3试剂盒检测凋亡信号活性。结果 MTT实验表明As2O3使MCF-7细胞存活率下降并呈现剂量依赖的量效关系;流式细胞测定结果证实As2O3对乳腺癌细胞的致凋亡作用;As2O3作用MCF-7细胞在2h内细胞出现AVD,但Caspase-3的活性无明显变化,直到作用MCF-7细胞6h后Caspase-3活性显著升高。结论 AVD是As2O3诱导乳腺癌细胞凋亡的早期事件。

Apoptotic Volume Decrease is the Early Event in Arsenic Trioxide Induced Apoptosis in Breast Cancer Cells

OBJECTIVE To investigate whether the apoptotic volume decrease is the early event in arsenic trioxide induced apoptosis in breast cancer MCF-7 cells. METHODS The effect of As₂O₃ on cell viability was assayed by methylthiazoletetrazolium(MTT). Apoptosis was analyzed by flow cytometry. Lag-time microphotography was employed to detect cell volume. The apoptotic signaling activity of Caspase-3 was detected by caspase assay kit. RESULTS MTT result showed As₂O₃ reduced the viability of MCF-7 cells in a concentration-dependent manner. Flow cytometry assay confirmed apoptosis resulted in As₂O₃-treated MCF-7 cells. Apoptotic volume decrease was observed within 2 h in As₂O₃ treated MCF-7 cells, but the caspase-3 activity had no significant change. The caspase-3 activity increased significantly until As₂O₃ treated MCF-7 cells for 6 h. CONCLUSION Apoptotic volume decrease is the early event in arsenic trioxide induced apoptosis in breast cancer cells.