黄芪皂甙诱导小鼠神经干细胞向神经元分化

目的：观察黄芪皂甙对体外培养的小鼠神经干细胞（NSCs）向神经元分化的影响.方法：采用机械分离、无血清传代培养法从胚胎（E14.5）小鼠室管膜前下区获得NSCs,观察不同浓度（20,40,60mg/L）黄芪皂甙在干预后不同时段（3,7d）的分化情况,通过免疫荧光检测神经元（β-Tubulin）,星形胶质细胞（GFAP）及少突胶质细胞（CC-1）的比例,并采用银杏内酯B（40mg/L）作为阳性对照,正常组为阴性对照,观察黄芪皂甙对小鼠NSCs分化的影响.结果：加入黄芪皂甙培养3,7d后,黄芪皂甙40mg/L及60mg/L组、银杏内酯B组分化为β-Tubulin（＋）神经元比例均显著高于正常对照组,黄芪皂甙两种浓度组间无统计学差异;银杏内酯B组分化为GFAP（＋）星形胶质细胞比例显著高于正常对照组,而黄芪皂甙对星形胶质细胞的分化比例无明显影响.结论：黄芪皂甙能促进NSCs定向分化为神经元,而银杏内酯B促进NSCs向神经元和星形胶质细胞分化.

Effects of Saponins of Astragalus on differentiation of neural stem cells

AIM： To observe the effects of Saponins of Astragalus（SA） of various concentrations on the differentiation of neural stem cells（NSCs）. METHODS： NSCs from the anterior subventricular zone of the embryonic mouse （ EI4.5 ） were generated by mechanical isolation and serum-free culture. To study the effects of different concentrations （20, 40, 60 mg/L ） of SA on the differentiation of the NSCs at different stages （ 3 and 7 d） , we adopted immunofluorescence to examine the proportion of the neuron（ 3-Tubulin）, astrocyte（GFAP） and oligodendrocyte （CC-I） by adding different concentrationsof SA. 40 mg/L Ginkgolide B （GKB） was employed as positive control of SA and normal medium was employed as negative control. RESULTS： The proportion of β-Tubulin positive neurons significantly increasedafter 3 and 7 d cuhure with SA of 40 and 60 mg/L and the GKB and no significant difference was observed between 40 and 60 mg/L of SA. The proportion of GFAP positive astrocytes increased significantly with the adding of GKB, while SA had no obvious effects on the differentiation of the astrocytes. CONCLUSION： Saponins of Astragalus specifically promotes NSCs to differentiate into neurons, while GKB promotes the NSCs to differentiate into both neurons and astrocytes.