| hPer1相互作用蛋白的筛选 |
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| 引用本文： | 冀治鸿,江舟,汪宇辉,鲁芳,刘延友,高梅,王正荣. hPer1相互作用蛋白的筛选[J]. 航天医学与医学工程, 2007, 20(3): 184-187 |
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| 作者姓名： | 冀治鸿 江舟 汪宇辉 鲁芳 刘延友 高梅 王正荣 |
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| 作者单位： | 四川大学华西医学中心基础与法医学院,生物医学工程研究室,四川成都,610041 |
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| 摘 要： | 目的 应用酵母双杂交系统筛选血液中与人体生物钟蛋白Per1相互作用的新蛋白.方法 以人Per1的basic HLH-PAS结构域为诱饵,在人血液cDNA文库中筛选与之相互作用的新蛋白.阳性克隆送测序后用生物信息学分析.结果 酵母双杂交文库营养缺陷筛选得到114个阳性克隆,β-半乳糖苷酶检测报告基因获得46个蓝色克隆.通过测序和生物信息学分析,其中之一能与Per1相互作用的蛋白为LSMD1.结论 通过酵母双杂交系统证明Per1能够与LSMD1发生相互作用.
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| 关 键 词： | 近日节律 酵母双杂交 Per1基因 LSMD1蛋白 相互作用蛋白 相互作用蛋白 筛选 Interacting Proteins 发生 阳性克隆 报告基因 酶检测 半乳糖苷 营养缺陷 杂交文库 酵母双杂交系统 结果 生物信息学分析 测序 人血液 诱饵 结构域 basic 方法 人体生物钟 |
| 文章编号： | 1002-0837（2007）03-0184-04 |
| 修稿时间： | 2006-11-02 |
Screening the Interacting Proteins of hPer1 |
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| JI Zhi-hong,JIANG Zhou,WANG Yu-hui,LU Fang,LIU Yan-you,GAO Mei,WANG Zheng-rong. Screening the Interacting Proteins of hPer1[J]. Space Medicine & Medical Engineering, 2007, 20(3): 184-187 |
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| Authors: | JI Zhi-hong JIANG Zhou WANG Yu-hui LU Fang LIU Yan-you GAO Mei WANG Zheng-rong |
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| Affiliation: | School of Basic and Forensic Medicine,Sichuan University , Chengdu Sichuan 610041 ,China |
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| Abstract: | Objective To find a new method for identifying interacting proteins of human Perl in the blood. Methods Using bHLH-PAS domain of hPerl as bait, a human blood cDNA library was screened and the proteins interacting with hPerl were searched. The positive clones were sequenced and analyzed by bioinformatics methods. Results Forty-six colonies were selected by the yeast two-hybrid system. LSMDI protein was obtained after verification. Conclusion It is proved that Perl can interact with LSMD1 by yeast two-hybrid system. |
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| Keywords: | circadian yeast two-hybrid Perl LSMD1 protein interacting proteins |
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