推拉动作对大鼠脑GFAP表达的影响

目的 观察推拉动作后大鼠脑星形胶质细胞中胶质纤维酸性蛋白(GFAP)表达的改变.方法 雄性SD大鼠44只,随机分为对照组、 10 Gz暴露组、推拉动作组,分别于暴露后不同时间灌注取脑,免疫组织化学染色观察脑GFAP的表达情况.结果 10 Gz暴露后6 h,顶叶皮层、海马、丘脑可见GFAP呈中等强度的阳性反应,阳性细胞数较对照组显著增多(P＜0.01),多为中等阳性细小型;暴露后1 d、2 d、4 d和6 d,GFAP阳性反应程度进一步增强,阳性细胞数进一步增多.推拉动作组暴露后各时间点,顶叶皮层、海马、丘脑GFAP呈较强的阳性反应,阳性细胞数较 10 Gz暴露组显著增多(P＜0.01),以强阳性肥大型为主.结论 10 Gz/3 min 暴露可引起大鼠顶叶皮层、海马、丘脑GFAP表达显著增加,而推拉动作组比 10 Gz暴露组引起脑组织GFAP的表达增加更明显.

Effects of Push-pull Maneuver on GFAP Expression of Rat Brain

Objective To explore influence of the push-pull G on glial fibrillary acidic protein(GFAP)expression in rat brain astrocytes.Methods Forty-four SD male rats were randomly divided into control group, 10 Gz exposure group and push-pull G group.The rats were respectively killed at different time after exposure and the GFAP expression in rat astrocytes was measured by immunohistochemistry staining.Results Compared with control group,GFAP positive reaction was medium strength,and the number of GFAP-positive cells increased significantly(P<0.01)in the parietal cortex,hippocampus and cerebral ganglion 6 h after 10 Gz exposure,most of them belong to medium positive thin type;1 d,2 d,4 d,and 6 d after 10 Gz exposure,the GFAP positive reaction increased further and the number of GFAP-positive cells increased further.Compared with 10 Gz exposure group,the GFAP positive reaction was stronger and the number of GFAP-positive cells increased significantly(P<0.01)at the same time after exposure to push-pull G,most of the GFAP-positive cells belong to strong positive fat type.Conclusion 10 Gz/3 min exposure may induce significant increase of GFAP expression in rat parietal cortex,hippocampus and cerebral ganglion.GFAP expression increases more significantly after exposure to push-pull G than after 10 Gz exposure.