三黄活络方对早期糖尿病肾病大鼠血糖,肾功能,SREBP-1c,SREBP-2c的影响

目的:观察三黄活络方对早期糖尿病肾病大鼠血糖,肾功能,固醇调节元件结合蛋白-1c(SREBP-1c),SREBP-2c的影响。方法:采用链脲佐菌素诱导的方法建立大鼠糖尿病模型,将糖尿病模型成功的大鼠随机分为模型组、阳性药组及三黄活络方低、中、高剂量组,其它组行切除大鼠右侧肾脏术后ig,阳性药组给予1.3 mg·kg-1盐酸贝那普利,三黄活络方低剂量组、中剂量组和高剂量组均给予中药颗粒,剂量分别为5,15,30 g·kg-1。正常组和模型组均ig给予蒸馏水,所有组均连续ig 8周。观察大鼠血糖(GLU),糖化血红蛋白(Hb A1c),血尿素氮(BUN),肌酐(SCr),尿蛋白(Pro),SREBP-lc,SREBP-2c的水平。结果:与正常组比较,模型组的GLU和Hb A1c均显著性升高(P0.01),阳性药组、各三黄活络组的GLU和Hb A1c也均显著性升高(P0.01);与模型组比较,阳性药组、各三黄活络组的GLU均降低(P0.01),阳性药组、中、高剂量组的Hb A1c均降低(P0.01),低剂量组的Hb A1c降低(P0.05)。与正常组比较,模型组的SCr,BUN,Pro,SREBP-1c及SREBP-2c均显著性升高(P0.01),阳性药组和各三黄活络组的SCr,BUN,Pro,SREBP-1c及SREBP-2c也均显著性升高(P0.01)。与模型组对比,阳性药组、各三黄活络组的SCr,BUN,Pro,SREBP-1c及SREBP-2c均较低(P0.01)。与阳性药组对比,低剂量组的SCr,BUN,Pro,SREBP-1c及SREBP-2c均较高(P0.01),中剂量组的的SCr,BUN,Pro,SREBP-1c及SREBP-2c均较高(P0.05)。结论:三黄活络方能改善糖尿病肾病大鼠SCr,BUN,Pro,SREBP-1c及SREBP-2c水平,对糖尿病肾病大鼠肾损伤有一定的保护作用。

Effect of Sanhuang Huoluo Formula on Blood Glucose, Renal Function, SREBP-1c and SREBP-2c of Diabetic Nephropathy Rats

Objective: To observe the effect of Sanhuang Huoluo formula on blood glucose, renal function, sterol regulating element binding protein-1c (SREBP-1c) and SREBP-2c of diabetic nephropathy rats. Method: The diabetic model was induced by streptozotocin. The rats in the successfully established diabetic model were randomly divided into the model group, the positive drug group and Sanhuang Huoluo formula low, medium and high doses groups. After other groups took the resection of the right kidney, the positive drug group was given 1.3 mg· kg^-1 of benazepril hydrochloride, Sanhuang adjustable low dose group, middle dose group and high dose group were orally given Chinese medicine granules with doses of 5, 15, 30 g· kg^-1, respectively. Both of the normal group and the model group mice were orally given distilled water, and all of the groups orally given drugs for 8 weeks in a row. Blood glucose (GLU), glycosylated hemoglobin (HbA1c), blood urea nitrogen (BUN), creatinine (SCr), urine protein (Pro), SREBP-lc, SREBP-2c in rats were observed. Result: Compared with the normal group, GLU and HbA1c of the model group were significantly higher (P<0.01), GLU and HbA1c of the positive drug group and Sanhuang Huoluo formula low, medium and high doses groups were also significantly higher (P<0.01). Compared with the model group, GLU of the positive drug group and Sanhuang Huoluo formula low, medium and high doses groups were lower (P<0.01), HbA1c of the positive drug group and Sanhuang Huoluo formula medium and high doses groups were lower (P<0.01), HbA1c of the Sanhuang Huoluo formula low dose group was lower (P<0.05). Compared with the normal group, SCr, BUN, Pro, SREBP-lc and SREBP-2c of the model group were significantly higher (P<0.01), and SCr, BUN, Pro, SREBP-lc and SREBP-2c of the positive drug group and Sanhuang Huoluo formula low, medium and high doses groups were also significantly higher (P<0.01). Compared with the model group, SCr, BUN, Pro, SREBP-lc and SREBP-2c of the positive drug group and Sanhuang Huoluo formula low, medium and high doses groups were lower (P<0.01). Compared with the positive drug group, SCr, BUN, Pro, SREBP-lc and SREBP-2c of the Sanhuang Huoluo formula low dose group were higher (P<0.01), and SCr, BUN, Pro, SREBP-lc and SREBP-2c of the Sanhuang Huoluo formula medium dose group were higher (P<0.05). Conclusion: Sanhuang Huoluo formula can improve level of SCr, BUN, Pro, SREBP-lc and SREBP-2c in diabetes nephropathy rats, with a certain protective effect on renal damage in rats of diabetic nephropathy rats.