接骨创伤膏联合中药离子导入对创伤性骨折愈合的影响

目的:探讨接骨创伤膏联合中药离子导入对骨折愈合的影响,明确该项联用技术是否有加强骨折愈合的作用及可能的机制。方法:将雄性健康新西兰兔160只随机等分为正常组,接骨创伤膏联合中药离子导入给药组(简称联合治疗组),接骨创伤膏组(仅给予接骨创伤膏,简称对照组),模型组4组,每组40只,除正常组外,均建立右侧桡骨骨折模型,以第2,4,6,8周4个时间点将每组动物随机等分成4小组,每组10只,联合治疗组所有动物右侧骨折部位给予接骨创伤膏联合中药离子导入治疗,接骨创伤膏组右侧骨折部位单纯给予接骨创伤膏治疗,正常组和模型组不予治疗。术后治疗的第2,4,6,8周4个时间组进行血清碱性磷酸酶(alkaline phosphatase,AKP)活性及骨钙素(bone gla protein,BGP)含量测定,骨密度,X射线影像学检查,组织切片和形态学检测。结果:与正常组比较,模型组血清AKP活性水平,BGP含量,断段骨密度,X射线片影像学积分明显降低(P0.01),病理学和形态学检测显示骨折较明显;与模型组比较,联合治疗组治疗的第2,4,6,8周,血清AKP活性水平及BGP含量较对照组显著升高(P0.05,P0.01),骨密度,X射线片影像学积分明显显高(P0.05,P0.01),病理学和形态学检测亦显示联合治疗组骨折愈合明显提前,各时间点两组间比较差异均有统计学意义(P0.05,P0.01)。结论:接骨创伤膏联合中药离子导入通过对骨折愈合的整个过程施加影响从而加速了骨折的愈合。

Effect of Jiegu Chuangshang Ointment Combined with Iontophoresis of Traditional Chinese Medicine on Traumatic Fracture Healing

Objective: To investigate the effect of Jiegu Chuangshang ointment combined with iontophoresis of traditional Chinese medicine on fracture healing, and define its effect in strengthening the healing of fracture and its possible mechanism. Method: Totally 160 male healthy New Zealand rabbits were divided randomly into blank control group (blank group for short), Jiegu Chuangshang ointment combined with iontophoresis of traditional Chinese medicine group (combined therapy group for short), Jiegu Chuangshang ointment control group (treated only with Jiegu Chuangshang ointment, control group for short) and model group, with 40 rabbits in each group. Except for the blank group, all of the other animals were included in the right radius fracture model and were subdivided randomly into 4 small groups at 4 time points-the 2th, 4th, 6th, 8th weeks, with 10 in each group. The combined therapy group was treated with Jiegu Chuangshang ointment combined with iontophoresis of traditional Chinese medicine, and control group only received the treat of Jiegu Chuangshang ointment, blank group and model group were not treated. At the 2th, 4th, 6th, 8th week, serum alkaline phosphatase (AKP) activity and bone gla protein (BGP) content were determined, and the bone mineral density test, X-ray imaging check, histological and morphological detection were completed. Result: Compared with the normal group, the model group showed significant lower serum AKP and BGP content, bone mineral density and X-ray imaging score (P<0.01) and obvious pathological and morphological detection results. Compared with the model group, at the 2th, 4th, 6th, 8th week after treatment, the activity level of serum AKP and BGP content of the combined therapy group was significantly higher (P<0.05, P<0.01). Besides, the bone mineral density, X-ray imaging score, histological sections and morphological detection also shows that the fracture healing in the combined therapy group was ahead of schedule (P<0.05, P<0.01), and the differences at each time point between the two groups were significant statistically (P<0.05, P<0.01). Conclusion: Jiegu Chuangshang ointment combined with iontophoresis of traditional Chinese medicine accelerate the healing of fracture by influencing the whole process of fracture healing.