c-myc转染对舌鳞癌细胞系Tca8113fas表达和凋亡的影响

背景与目的：恶性肿瘤的发生不仅与细胞的异常增殖有关,还与细胞的凋亡异常有关,本实验目的在于研究原癌基因c-myc(cellular avian myelocytoma virus)介导舌鳞癌细胞凋亡的可能性和分子机制。方法：脂质体法将含c-myc的重组质粒转染到舌鳞癌细胞Tca8113,G418筛选阳性克隆,RT－PCR检测转染前后fas(fatty acid synthase）表达水平,TUNEL法检测细胞凋亡水平。结果：转染后fas mRNA表达上调,正常培养条件下,转染前后细胞凋亡指数无明显改变,低浓度血清条件下细胞凋亡指数增加,结论：低营养状态下c-myc可以介导舌鳞癌细胞凋亡,凋亡机制可能与上调fas mRNA表达有关。

Effect of c-myc transfection on fas expression and apoptosis in oral squamous cell carcinoma cell line Tca8113

Background and Objective:The process of carcinogenesis is not only correlated to the abnormality of cell proliferation but also to the change of normal cell apoptosis. The aim of this study was to investigate the mechanism of apoptosis induced by c myc(cellular avian myelocytoma virus) gene in oral squamous cell carcinoma cell line Tca8113. Methods:Plasmid including c myc gene was transfected into Tca8113 cell by using lipofectamine kit. The cells were selected by G418. The expression of fas(fatty acid synthase) gene in transfected and non transfected Tca8113 cell was detected by RT PCR. Apoptosis of Tca8113 cell was determined by TUNEL. Results:Up regulation of fas expression was found in c myc transfected Tca8113 cell. The increased apoptosis index (AI) could only be found under the condition of low serum concentration. Conclusions:c myc could mediate apoptosis of oral squamous cell carcinoma in certain condition. This might be related with the up regulation of fas expression.