| SARS冠状病毒S蛋白S1区多肽与SARS病人血清的免疫反应 |
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| 引用本文： | 张嘉杰,吴少瑜,徐伟,侯金林,王战会,姜世勃,吴曙光.SARS冠状病毒S蛋白S1区多肽与SARS病人血清的免疫反应[J].南方医科大学学报,2004,24(7):789-790. |
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| 作者姓名： | 张嘉杰 吴少瑜 徐伟 侯金林 王战会 姜世勃 吴曙光 |
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| 作者单位： | 第一军医大学药物研究所,广东,广州,510515;第一军医大学南方医院传染科,广东,广州,510515;美国纽约血液中心 |
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| 基金项目： | 广东省科技厅科研攻关项目SARS专项基金~~ |
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| 摘 要： | 目的 测定根据计算机预测的SARS冠状病毒S蛋白S1区的抗原表位多肽与SARS病人血清的免疫反应,以寻找和确定SARS相关冠状病毒的中和抗原表位。方法 采用多肽合成仪合成SARS冠状病毒S蛋白S1区的多肽,并用ELISA方法检测合成的多肽与SARS病人血清的免疫反应。结果 合成的8个多肽与SARS病人血清免疫反应的光密度值是其与正常人血清免疫反应的光密度值的1.5～2.6倍。结论 合成的多肽与SARS病人血清的免疫反应较低,提示这些多肽是否为SARS冠状病毒的抗原表位还要进一步研究。
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| 关 键 词： | 冠状病毒 SARS 表位 多肽疫苗 免疫反应 |
| 文章编号： | 1000-2588(2004)07-0789-02 |
| 修稿时间： | 2003年11月18 |
Immunological reaction between the peptides from S1 domain of SARS coronavirus S-protein and the serum from SARS patients |
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| ZHA.NG Jia-jie,WU Shao-yu,XU Wei,HOU Jin-lin,WANG Zhan-hui,JIANG Shi-bo,WU Shu-guang Institute of Pharmaceutics Science.Immunological reaction between the peptides from S1 domain of SARS coronavirus S-protein and the serum from SARS patients[J].Journal of Southern Medical University,2004,24(7):789-790. |
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| Authors: | ZHANG Jia-jie WU Shao-yu XU Wei HOU Jin-lin WANG Zhan-hui JIANG Shi-bo WU Shu-guang Institute of Pharmaceutics Science |
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| Institution: | ZHA.NG Jia-jie~1,WU Shao-yu~1,XU Wei~1,HOU Jin-lin~2,WANG Zhan-hui~2,JIANG Shi-bo~3,WU Shu-guang~1 ~1Institute of Pharmaceutics Science,~2Department of Infectious Diseases,Nanfang Hhospital,First Military Medical University,Guangzhou 510515,China, ~3New York Blood Center,310E.67th Street,New York,USA |
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| Abstract: | Objective To examine the immunological reactions between the peptides of SARS coronavirus (SARS-Cov) S-protein and the serum of SARS patients for identifying the SARS-Cov epitope. Methods The peptides from S1 domain of SARS-Cov S-protein were synthesized by peptide synthesizer, and the immunological reaction of the peptides with the serum of SARS patients were examined by means of ELISA. Results The optical density value of the immunological reaction of synthesized 8 peptides with SARS patient serum was 1.5-2.6 times higher than that with normal serum. Conclusion The 8 peptides from S1 domain of S protein appear to have low immunogenicity to the serum of SARS patients, indicating that these peptides may not be the epitope of the SARS-Cov. |
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| Keywords: | coronavirus severe acute respiratory syndrome epitope peptide vaccine immune reaction |
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