| 新生期母婴分离对幼鼠内脏痛敏感性及脊髓Fos蛋白表达的影响 |
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| 引用本文： | 林滨榕,吴斌,卓玲,陈兢芳,杨燕珍,张睿. 新生期母婴分离对幼鼠内脏痛敏感性及脊髓Fos蛋白表达的影响[J]. 新生儿科杂志, 2010, 0(2) |
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| 作者姓名： | 林滨榕 吴斌 卓玲 陈兢芳 杨燕珍 张睿 |
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| 作者单位： | 福建医科大学附属第一医院儿科; |
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| 基金项目： | 福建省卫生厅青年科研课题(2008-2-22); 福建省教育厅科技项目(JB08094); 福建医科大学第一临床医学院学科带头人培养对象专项基金(JXK200716) |
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| 摘 要： | 目的探讨新生期母婴分离(MS)对幼鼠内脏痛觉敏感性及脊髓背角Fos蛋白表达的影响。方法按析因设计,32只SD新生大鼠分成4组,每组8只,A1B1组为MS组并在6周龄时接受结直肠扩张刺激(CRD),A1B2组为MS组,6周龄未给予CRD,A2B1组未MS在6周龄接受CRD,A2B2组未MS,且6周龄未给予CRD。A1B1组、A2B1组6周龄时进行CRD下腹壁撤退反射(AWR)评分和痛阈测定;A1B1组、A2B1组幼鼠在CRD刺激后2h和A1B2组、A2B2组幼鼠一起处死,留取L6至S2脊髓,应用免疫组织化学染色及计算机图像分析系统进行脊髓Fos阳性(FLI)细胞数半定量分析。结果CRD压力为20、40、60、80mmHg时,A1B1组AWR评分分别为(2.2±0.7)、(3.2±0.6)、(3.8±0.4)、(4.0±0.0)分,A2B1组分别为(1.0±0.8)、(2.5±0.3)、(3.3±0.3)、(3.9±0.2)分;CRD为20、40、60mmHg时,两组差异有统计学意义。A1B1组和A2B1组痛阈值分别为(17.9±8.9)mmHg、(33.5±1.9)mmHg,差异有统计学意义(P=0.000)。...
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| 关 键 词： | 痛觉过敏 原癌基因蛋白质c-fos 母婴分离 大鼠 |
Effect of neonatal maternal separation on visceral hyperalgesia in the developing rats and role of fos abnormal expression in spinal cord |
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| LIN Bin-rong,WU Bin,ZHUO Ling,CHEN Jing-fang,YANG Yan-zhen,ZHANG Rui. Effect of neonatal maternal separation on visceral hyperalgesia in the developing rats and role of fos abnormal expression in spinal cord[J]. The Journal of Neonatology, 2010, 0(2) |
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| Authors: | LIN Bin-rong WU Bin ZHUO Ling CHEN Jing-fang YANG Yan-zhen ZHANG Rui |
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| Affiliation: | LIN Bin-rong,WU Bin,ZHUO Ling,CHEN Jing-fang,YANG Yan-zhen,ZHANG Rui. Department of Pediatrics,The First Affiliated Hospital of Fujian Medical University Fuzhou 350005,China |
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| Abstract: | Objective To explore the effect of maternal separation (MS) stimulation in neonatal period on visceral sensitivity of pain in developing rats. Observeing the Fos expression at dorsal horn of lumborsacral spinal cord in rats to investigate the mechanism of highly visceral pain sensitivity. Methods According to the factorial design,32 SD rats were divided into four groups of 8 each. Group A1B1 was experimental group and subjected to colorectal distention (CRD) at 6-week age,yet group A1B2 were not imposed on ... |
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| Keywords: | Hyperalgesia Proto-oncogene proteins c-fos Maternal separation Rats |
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