Preparation and evaluation of poly(ester-urethane) urea/gelatin nanofibers based on different crosslinking strategies for potential applications in vascular tissue engineering |
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Authors: | Yao Wang Tonghe Zhu Haizhu Kuang Xiaoning Sun Jingjing Zhu Yu Shi Chunsheng Wang Xiumei Mo Shuyang Lu Tao Hong |
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Institution: | Department of Cardiovascular Surgery, Zhongshan Hospital, Fudan University, The Shanghai Institute of Cardiovascular Diseases, Shanghai 200032 People''s Republic of China.; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620 People''s Republic of China, |
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Abstract: | Due to the brittleness of gelatin, the resulting absence of mechanical performance restricts its applications in vascular tissue engineering. In this research, the fabrication of poly(ester-urethane) urea/gelatin (PU75) nanofibers via an electrospinning technique, followed by different crosslinking methods, resulted in the improvement of its mechanical properties. Poly(ester urethane) urea (PEUU) nanofibrous scaffolds and PU75-based nanofibrous scaffolds were characterized using scanning electron microscopy (SEM), attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, wide-angle X-ray diffraction (WAXRD), a mechanical properties test, a cytocompatibility assay, a hemolysis assay, and a histological analysis. Water contact angle (WCA) tests confirmed that the PU75-GA (PU75 nanofibers crosslinked with glutaraldehyde vapor) nanofibrous scaffold surfaces became more hydrophilic compared with other crosslinked nanofibrous scaffolds. The results show that the PU75-GA nanofibrous scaffold exhibited a combination of excellent mechanical properties, suitable pore diameters, hydrophilic properties, good cytocompatibility, and reliable hemocompatibility. Overall, PU75-GA nanofibers may be a potential scaffold for artificial blood vessel construction.SEM micrographs of the PEUU nanofibrous membrane, PU75 nanofibrous membrane, PU75-DT nanofibrous membrane, PU75-GA nanofibrous membrane, and PU75-E/N nanofibrous membrane and magnified 1000, 5000, and 10 000 times, respectively. |
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