Interaction mechanism of olaparib binding to human serum albumin investigated with NMR relaxation data and computational methods |
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| Authors: | Yuanming Zhai Pengchi Deng Xiaoyan Wang Chunchun Zhang Ruixue Gan Na Gan Qiaomei Sun Hui Li |
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| Affiliation: | Analytical & Testing Center, Sichuan University, Chengdu 610064 China.; School of Chemical Engineering, Sichuan University, Chengdu 610065 China |
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| Abstract: | The interaction mechanism between olaparib (OLA) and human serum albumin (HSA) has been investigated using experimental and computational techniques. An NMR relaxation approach based on the analysis of proton selective and non-selective spin–lattice relaxation rates at different temperatures can provide quantitative information about the affinity index and the thermodynamic equilibrium constant of the OLA–HSA system. The affinity index and the thermodynamic equilibrium constant decreased as temperature increased, indicating that the interactions between OLA and HSA could be weakened as temperature increased. Molecular docking and dynamics simulations revealed that OLA stably bound to subdomain II (site 1), and OLA could induce the conformational and micro-environmental changes in HSA. CD results suggested that α-helix content decreased after OLA was added, demonstrating that OLA affected the secondary structure of HSA.The interaction mechanism between olaparib (OLA) and human serum albumin (HSA) has been investigated using experimental and computational techniques. |
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