Specific and sensitive imaging of basal cysteine over homocysteine in living cells |
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| Authors: | Longxue Nie Bingpeng Guo Congcong Gao Shaowen Zhang Jing Jing Xiaoling Zhang |
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| Affiliation: | Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photo-electronic/Electro-photonic Conversion Materials, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081 P. R. China, |
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| Abstract: | Biological thiols play important roles in maintaining appropriate redox status of organisms. Accepting the challenge to differentiate structurally similar cysteine (Cys) and homocysteine (Hcy), we have successfully developed a miniature synthetic turn-on fluorescent probe based on 6-(2-benzothiazolyl)-2-naphthalenol for Cys. This probe is able to specifically react with Cys to yield its naphthalenol derivative, accompanied by remarkable green fluorescence enhancement with a detection limit of 14.8 nM. Besides, this probe displays much greater selectivity for Cys over other biological thiols, including homocysteine (Hcy) and glutathione (GSH). Practically, good cell permeability and low cytotoxicity make it suitable for monitoring basal Cys in living cells.A turn-on fluorescent probe conjugating with a reactive acrylate for visualization of basal Cys specifically in living cells was developed. |
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