“Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex |
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| Authors: | Miao Wang Minmin Li Jia Lu Bei Fan Yan He Yatao Huang Fengzhong Wang |
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| Affiliation: | Key Laboratory of Quality & Safety Risk Assessment on Agro-Products Processing (Beijing), Ministry of Agriculture, Beijing 100193 China.; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193 China ; Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000 China |
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| Abstract: | Herein, we report novel “off–on” fluorescent sensing of organophosphate pesticides using a carbon dot (CD)–Au(iii) complex/acetylcholinesterase (AChE) system. The above sensor utilizes the quenching of CD fluorescence by Au(iii) and its subsequent recovery by thiocholine, which is generated by AChE-catalyzed hydrolysis of acetylthiocholine (ATCh) and effectively scavenges Au(iii). In the presence of organophosphates, the catalytic activity of AChE is inhibited, allowing these species to be quantified based on the decreased recovery of CD fluorescence intensity. The developed sensor was used to analyze a real pesticide-spiked sample (4.48 μM), achieving a recovery of 99.85% and exhibiting a linear response range of 0.45–44.80 μM.Herein, we report novel “off–on” fluorescent sensing of organophosphate pesticides using a carbon dot (CD)–Au(iii) complex/acetylcholinesterase (AChE) system. |
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