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3β-Hydroxy-Δ5-steroidal congeners from a column fraction of Dendronephthya puetteri attenuate LPS-induced inflammatory responses in RAW 264.7 macrophages and zebrafish embryo model
Authors:I. P. Shanura Fernando  Won Woo Lee  Thilina U. Jayawardena  Min-Cheol Kang  Yong-Seok Ann  Chang-ik Ko  Young Jin Park  You-Jin Jeon
Affiliation:Department of Marine Life Science, Jeju National University, Jeju 63243 Republic of Korea, +82-64-754-3475, +821-045723624 ; Choung Ryong Fisheries Co. Ltd., 7825, Iljudong-ro, Namwon-eup, Seogwipo-si, Jeju-do Korea ; Department of Family Medicine, College of Medicine, Dong-A University, Busan 602-715 Republic of Korea
Abstract:Bioactive compounds from marine organisms and their action mechanisms have provided new insights into medicinal and natural product research. Here, we report the identification of 3β-hydroxy-Δ5-steroidal congeners from a purified column fraction (DPCMH24) of the soft coral Dendronephthya puetteri harvested from Jeju, South Korea. DPCMH24 exerted strong anti-inflammatory effects through a dose-dependent decrease in the levels of nitric oxide (NO) in LPS-induced RAW 264.7 macrophages (IC50 value = 6.54 ± 0.38 μg mL−1). Further, DPCMH24 attenuated the levels of PGE2 and the pro-inflammatory cytokines, TNF-α, IL-1β, and IL-6. The above effects were mediated via the inhibition of nuclear factor κB activation and mitogen-activated protein kinase pathways. In vivo evaluation indicated that DPCMH24 reduced NO, iNOS, COX-2, ROS production and cell death in LPS-induced zebrafish embryos, confirming its anti-inflammatory potential. The constituent compounds were identified by GC-MS/MS analysis. These findings suggest that the steroidal congeners from D. puetteri may offer ample therapeutic potential against LPS-induced inflammation.

Bioactive compounds from marine organisms and their action mechanisms have provided new insights into medicinal and natural product research.
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