Screening of peptide probe binding to particulate matter with a high metal content

Particulate matter (PM) is becoming an increasing health concern and there is a need to develop detection methods to keep its harmful effects in check. Generation of reactive oxygen species (ROS) by PM is often associated with metal compounds, hence our aim is to screen for a peptide probe towards improved collection and the detection of PM having a high metal content. Peptides are putative recognition molecules due to their versatility and ease of modification to enhance their binding selectivities. PM binding peptides were screened using the peptide array and different binding behaviors in terms of different spot colors (yellow, mixed and gray), indicating the different composition of bound PMs, were observed. The strongest binding peptides were identified as follows: NHVNTNYYPTLH (gray), NGYYPHSHSYHQ (mixed) and HHLHWPHHHSYT (yellow), with relative binding ratios of 125%, 144% and 136%, in comparison with WQDFGAVRSTRS, a peptide screened from a phage display in our previous study. Inductively coupled plasma mass spectrometry (ICPMS) analyses revealed that Co, Ni and Zn content in the PM bound to the HHLHWPHHHSYT peptide spot were respectively 12.5, 15.8 and 7.8 times that of the PM bound to no peptide spot, suggesting this peptide probe is applicable to collect PM with a high metal content.

Using peptide array, peptides binding to particulate matter with high metal content were screened and characterized by focusing on the different spot colors (yellow, mixed and gray).