| 川芎嗪对顺铂耳毒性保护作用的实验研究 |
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| 引用本文： | 孙宪昌,张文娟,康颂建.川芎嗪对顺铂耳毒性保护作用的实验研究[J].泰山医学院学报,2010,31(7):483-486. |
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| 作者姓名： | 孙宪昌 张文娟 康颂建 |
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| 作者单位： | 1. 泰山医学院生理学教研室,山东,泰安,271000
2. 泰山医学院附属泰山医院,山东,泰安,271000 |
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| 基金项目： | 泰山医学院青年科研计划项目 |
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| 摘 要： | 目的探讨活血化瘀中药川芎嗪是否对顺铂的耳毒性具有保护作用。方法将听力正常的30只豚鼠随机分为3组,每组10只。对照组：生理盐水3 ml/（kg.d）腹腔注射6天;顺铂组：顺铂3 mg/（kg.d）腹腔注射6天;顺铂加川芎嗪组：先腹腔注射川芎嗪140 mg/（kg.d）3天,从第4天起腹腔注射川芎嗪140 mg/（kg.d）后再注射顺铂3 mg/（kg.d）6天。给药前后通过听觉脑干诱发电位（ABR）检测豚鼠听功能的变化;然后在麻醉状态下断头取耳蜗,半数标本切片后用DNA末端转移酶介导的缺口末端标记法（TUNEL）检测耳蜗内细胞凋亡情况;半数标本进行耳蜗基底膜铺片观察毛细胞形态及酶学变化。结果顺铂组用药后ABR阈值升高,毛细胞受损,琥珀酸脱氢酶（SDH）活性减弱,有部分毛细胞凋亡,与对照组及川芎嗪组比较差异具有统计学意义（P〈0.05）。顺铂加川芎嗪组用药后ABR阈值升高,毛细胞受损较轻,凋亡细胞较少,与顺铂组比较差异有统计学意义（P〈0.05）。结论川芎嗪能降低顺铂的耳毒性。
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| 关 键 词： | 顺铂 耳毒性 川芎嗪注射液 毛细胞 凋亡 |
The study on protection of ligustrazine against cisplatin-induced ototoxicity |
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| SUN Xian-chang,ZHANG Wen-juan,KANG Song-jian.The study on protection of ligustrazine against cisplatin-induced ototoxicity[J].Journal of Taishan Medical College,2010,31(7):483-486. |
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| Authors: | SUN Xian-chang ZHANG Wen-juan KANG Song-jian |
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| Institution: | SUN Xian-chang1,ZHANG Wen-juan2,KANG Song-jian1 (1.Dept.of Physiology,Taishan Medical University,Taian 271000,China,2.Taian Central Hospital,China) |
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| Abstract: | Objective: To explore the protective effect of ligustrazine against cisplatin-induced ototoxicity.Methods: Thirty adult guinea pigs were divided into three groups.Group A received saline as controls.Group B received cisplatin(3 mg/kg) injections for 6 consecutive days.Group C first received ligustrazine(140 mg/kg) for 3 days,and then received both cisplatin(3 mg/kg) and ligustrazine(140 mg/kg) for 6 consecutive days.Both ears of all the animals were tested with auditory brain stem response(ABR) before and o... |
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| Keywords: | cisplatin ototoxicity ligustrazine hair cells apopotosis |
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