川芎嗪对烫伤豚鼠血管内皮细胞保护作用的实验研究

目的：探讨川芎嗪对烧伤早期血管内皮细胞的保护作用。方法：用豚鼠３０％ＴＢＳＡⅢ度烫伤为模型，分为复苏组（ｎ＝４８）、治疗组（ｎ＝４８）和伤前对照（ｎ＝８）。复苏组在烧伤后立即给予平衡液复苏，治疗组在复苏同时给予川芎嗪。分别在伤前、伤后２、４、８、１２、２４和４８小时，测定血浆内皮素（ＥＴ）、一氧化氮（ＮＯ）；在伤后４和８小时取回结肠动脉中段作透射电镜观察。结果：治疗组各时相点ＥＴ水平较复苏组低（Ｐ〈０．０５，Ｐ〈０．０１），ＮＯ水平无显著性差异，两组ＥＴ／ＮＯ比值比较，有显著性差异（Ｐ〈０．０５）。病理显示，治疗因管内皮细胞损害明显轻于复苏组。结论：川芎嗪对烧伤早期血管内皮细胞有保护作用。

ExperimentalObservation of the Protective Effect of Ligustrazine on Vascular Endothelial Cells ofScalded Guinea Pig at the Early Stage

Objective: To study the protective effect of ligustrazine on vascular endothelial cells (VEC) in the early stage of burn. Method: Guinea pigs were scalded to 30%TBSA full-thickness and were divided into treatment group (n=48) and resuscitation group (n=48). And in control group (n=8), the animals were not scalded. In resuscitation group, animals were given balanced fluid for resuscitation, while in treatment group, in addition to balanced fluid, ligustrazine was administered. Plasma endothelin and nitric oxide levels were determined before injury and at 2, 4, 8, 12, 24 and 48 hr post injury. At 4 and 8 hr post injury, morphological changes of VEC of arteria ileocolia were examined under a transmission electron microscope. Results: The plasma ET level in the treatment group was significantly lower than that of the resuscitation group (P<0.05, P<0.01). The difference in serum nitric oxide level was not significant. The difference of ET/NO ratio between the two groups was significant (P<0.05). Pathological examination showed that in treatment group, vascular endothelial cell injury was significantly less than that of the resuscitation group. Conclusion: Ligustrazine has protective effect on the vascular endothelial cell at early stage of burn. [