中国妇女生殖道湿疣与人乳头瘤病毒关系的基因与亚显微结构研究

核酸杂交发现,中国妇女宫颈癌与人乳头瘤病毒(HPV)-16关系密切,而湿疣则与HPV-11关系更为密切。在宫颈癌细胞中,HPV-DNA整合在癌细胞基因组中;而湿疣细胞中的HPV则游离存在于宿主细胞基因组外。电镜观察发现,在杂交呈阳性的癌细胞中,核内染色质间颗粒。染色质周围颗粒及核内小体多见。但未见HPV颗粒;而在湿疣组织中,除上述核内染色质颗粒及核内小体外,在部分病例中可见HPV颗粒。此外,在湿疣细胞还发现一种电子致密团块结构。提出不能仅凭疣细胞中有凹空细胞(koilocyte),便诊断HPV感染。

Molecular and Ultrastructural Study on the Relationship between Human Papillomavirus and Female Genital Condyloma in China

From the results of DNA hybridization, it has been shown that there is a close relationship between HPV 16 and human uterine cervical carcinoma in China, and also that the viral DNA is integrated into the cancer cell genome. Genital condyloma revealed a high positive hybridization rate with HPV 11 DNA, and the viral DNA presented as an episome apart from the cell genome. Under the electron microscope, the inter and perichromatin granules as well as nuclear bodies were observed in all biopsies with positive hybridization, but no HPV particle was found in cancer cells, besides these two kinds of chromatin granules. Human papillomavirus nucleocapsid particles were observed in the nuclei of some genital condyloma cells. Electron-dense matrix aggregates of different sizes were seen in the perinuclear cytoplasm and in the nuclei. This kind of structure may be related to the process of HPV maturity, but its nature remains to be studied.